Rabbit Anti-ATP5F1A Recombinant Antibody (
5G11) (V2LY-0725-LY979)

Name: Rabbit Anti-ATP5F1A Recombinant Antibody (5G11)
SKU: V2LY-0725-LY979
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ATP5F1A Recombinant Antibody (5G11) (V2LY-0725-LY979) in WB

Western Blot
Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, Mouse Heart tissue, Mouse Brain tissue
All lanes: ATP5F1A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa

Rabbit Anti-ATP5F1A Recombinant Antibody (5G11) (V2LY-0725-LY979) in IHC

IHC image of V2LY-0725-LY979 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-ATP5F1A Recombinant Antibody (5G11) (V2LY-0725-LY979) in IHC

IHC image of V2LY-0725-LY979 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

5G11

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human ATP5A1.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ATP Synthase F1 Subunit Alpha

Entrez Gene ID

498

UniProt ID

P25705

Function

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed:30146159).

Biological Process

ATP biosynthetic process Source: CAFA
ATP synthesis coupled proton transport Source: GO_Central
Cristae formation Source: Reactome
Lipid metabolic process Source: UniProtKB
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Negative regulation of endothelial cell proliferation Source: UniProtKB
Positive regulation of blood vessel endothelial cell migration Source: CAFA

Cellular Location

Mitochondrion; Mitochondrion inner membrane; Cell membrane. Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951).

Involvement in disease

Combined oxidative phosphorylation deficiency 22 (COXPD22): A mitochondrial disorder characterized by intrauterine growth retardation, microcephaly, hypotonia, pulmonary hypertension, failure to thrive, encephalopathy, and heart failure.
Mitochondrial complex V deficiency, nuclear type 4 (MC5DN4): A mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid.

PTM

The N-terminus is blocked.
Acetylated on lysine residues. BLOC1S1 is required for acetylation.

Lines, M. A., Cuillerier, A., Chakraborty, P., Naas, T., Duque Lasio, M. L., Michaud, J., ... & Geraghty, M. T. (2021). A recurrent de novo ATP5F1A substitution associated with neonatal complex V deficiency. European Journal of Human Genetics, 1-6.

Feichtinger, R. G., Schäfer, G., Seifarth, C., Mayr, J. A., Kofler, B., & Klocker, H. (2018). Reduced levels of ATP synthase subunit ATP5F1A correlate with earlier-onset prostate cancer. Oxidative medicine and cellular longevity, 2018.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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