What applications is this antibody validated for?

The Mouse Anti-ATP5F1A Recombinant Antibody (15H4C4) (CAT: CBMAB-1167-CN) is validated for use in Western Blotting (WB), Immunofluorescence (IF), and Immunohistochemistry (IHC). Based on the target protein's characteristics and common antibody applications, it may also be suitable for Flow Cytometry (FCM) and ELISA, although specific validation data for these applications should be confirmed on the product datasheet or by contacting Creative Biolabs directly.

What is the recommended working dilution for this antibody?

Recommended working dilutions can vary depending on the specific application and experimental conditions (e.g., sample type, detection method). Please refer to the product datasheet for the recommended starting dilutions for WB, IF, and IHC. Optimization may be required to achieve optimal results in your specific experimental system.

Mouse Anti-ATP5F1A Recombinant Antibody (15H4C4) (CBMAB-1167-CN)

See all ATP5F1A antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

15H4C4

Application

WB, ICC, IF, IP, IHC, FC

Immunogen

Bovine Complex V.

Host Species

Mouse

Specificity

Human, Cattle, Fruit fly, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml
IF(ICC)	1-2 µg/ml
IHC	1 µg/ml
FC	1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C. Do not freeze.

More Infomation

Target

Full Name

ATP Synthase F1 Subunit Alpha

Introduction

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

Entrez Gene ID

Human	498
Mouse	11946
Rat	65262
Cattle	282578

UniProt ID

Human	P25705
Mouse	Q03265
Rat	P15999
Cattle	P19483

Alternative Names

OMR; ORM; ATPM; MOM2; ATP5A; hATP1; ATP5A1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m

Function

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed:30146159).

Biological Process

ATP biosynthetic process Source: CAFA
ATP synthesis coupled proton transport Source: GO_Central
Cristae formation Source: Reactome
Lipid metabolic process Source: UniProtKB
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Negative regulation of endothelial cell proliferation Source: UniProtKB
Positive regulation of blood vessel endothelial cell migration Source: CAFA

Cellular Location

Mitochondrion; Mitochondrion inner membrane; Cell membrane. Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951).

Involvement in disease

Combined oxidative phosphorylation deficiency 22 (COXPD22): A mitochondrial disorder characterized by intrauterine growth retardation, microcephaly, hypotonia, pulmonary hypertension, failure to thrive, encephalopathy, and heart failure.
Mitochondrial complex V deficiency, nuclear type 4 (MC5DN4): A mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid.

PTM

The N-terminus is blocked.
Acetylated on lysine residues. BLOC1S1 is required for acetylation.

Lines, M. A., Cuillerier, A., Chakraborty, P., Naas, T., Duque Lasio, M. L., Michaud, J., ... & Geraghty, M. T. (2021). A recurrent de novo ATP5F1A substitution associated with neonatal complex V deficiency. European Journal of Human Genetics, 1-6.

Feichtinger, R. G., Schäfer, G., Seifarth, C., Mayr, J. A., Kofler, B., & Klocker, H. (2018). Reduced levels of ATP synthase subunit ATP5F1A correlate with earlier-onset prostate cancer. Oxidative medicine and cellular longevity, 2018.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ATP5F1A Recombinant Antibody (15H4C4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Rabbit Anti-ATP5F1A Recombinant Antibody (EG302) (CAT#: CBMAB-EN333-LY)

Rabbit Anti-ATP5F1A Recombinant Antibody (555) (CAT#: CBMAB-AP489LY)

Mouse Anti-ATP5F1A Recombinant Antibody (CBT3588) (CAT#: V2LY-0625-LY1732)

Mouse Anti-ATP5F1A Recombinant Antibody (CBT2513) (CAT#: V2LY-0625-LY1733)

Mouse Anti-ATP5F1A Recombinant Antibody (7F9BG1) (CAT#: CBMAB-1168-CN)

Mouse Anti-ATP5F1A Recombinant Antibody (12F4AD8AF8) (CAT#: CBMAB-A4040-YC)

Mouse Anti-ATP5F1A Recombinant Antibody (7H10BD4F9) (CAT#: CBMAB-A4045-YC)

Mouse Anti-ATP5F1A Recombinant Antibody (CBLNA-177) (CAT#: CBMAB-1169-CN)

Mouse Anti-ATP5F1A Recombinant Antibody (51) (CAT#: CBMAB-A4043-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry