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Mouse Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (V2-443454) (PTM-CBMAB-0030YC)

Provided herein is a Mouse monoclonal antibody against AKT1 (Thr308)/AKT2 (Thr309)/AKT3 (Thr305). The antibody can be used for immunoassay techniques, such as WB.
See all AKT1/AKT2/AKT3 antibodies
Published Data
Mouse Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (L32A4) in IHC
pAkt expression in primary afferents of MA rats and colocalization with ATF-3. a-c Fluorescent microscope photo images depicting single immunolabeling for ATF-3 (red nuclei pointed with white arrows) ( a ), pAkt (green cytoplasms pointed with white arrows) ( b ), and double immunolabeled cells (red nuclei and green cytoplasms pointed with white arrows) ( c ) in a L 5 ganglia from a 4d MA rat. Scale bar represents 50 m. d Quantification of the percentage (%) of single labeled cells for pAkt (pAkt-IR cells) in the total DRG neuronal population, in L 5 ipsilateral DRGs, revealed no statistically significant differences among the experimental groups. e Percentage (%) of colocalization between ATF-3 and pAkt did not show any significant difference during disease progression. All values shown as mean 8 SEM. One-way ANOVA followed by Bonferroni's test. n = 5 rats for each of the MA experimental groups and n = 4 rats for the control group. ...View More
Nascimento, D., Pozza, D. H., Castro-Lopes, J. M., & Neto, F. L. (2011). Neuronal injury marker ATF-3 is induced in primary afferent neurons of monoarthritic rats. Neurosignals, 19(4), 210-221.
Mouse Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (L32A4) in Immunoblot
Immunoblot analysis of pThr308-Akt and Akt in LV of WT-SED, WT-ET, KO-SED and KO-ET female mice. ...View More
Støle, T. P., Lunde, M., Shen, X., Martinsen, M., Lunde, P. K., Li, J., ... & Carlson, C. R. (2022). The female syndecan-4−/− heart has smaller cardiomyocytes, augmented insulin/pSer473-Akt/pSer9-GSK-3β signaling, and lowered SCOP, pThr308-Akt/Akt and GLUT4 levels. Frontiers in Cell and Developmental Biology, 10, 908126.
Mouse Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (L32A4) in WB
Akt threonine 308 phosphorylation in normoxic and hypoxic NTC and mimic 193a cells was assessed by Western analyses and normalized to total Akt. ...View More
Fuhrmann, D. C., & Brüne, B. (2022). miR-193a-3p increases glycolysis under hypoxia by facilitating akt phosphorylation and PFKFB3 activation in human macrophages. Cellular and Molecular Life Sciences, 79(2), 89.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Monkey
Clone
V2-443454
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AKT1/AKT2/AKT3
Introduction
AKT is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration.
Alternative Names
AKT 1; AKT2; AKT3
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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