Mouse Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (V2-443454) (PTM-CBMAB-0030YC)

Name: Mouse Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (V2-443454)
SKU: PTM-CBMAB-0030YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (L32A4) in IHC

pAkt expression in primary afferents of MA rats and colocalization with ATF-3. a-c Fluorescent microscope photo images depicting single immunolabeling for ATF-3 (red nuclei pointed with white arrows) ( a ), pAkt (green cytoplasms pointed with white arrows) ( b ), and double immunolabeled cells (red nuclei and green cytoplasms pointed with white arrows) ( c ) in a L 5 ganglia from a 4d MA rat. Scale bar represents 50 m. d Quantification of the percentage (%) of single labeled cells for pAkt (pAkt-IR cells) in the total DRG neuronal population, in L 5 ipsilateral DRGs, revealed no statistically significant differences among the experimental groups. e Percentage (%) of colocalization between ATF-3 and pAkt did not show any significant difference during disease progression. All values shown as mean 8 SEM. One-way ANOVA followed by Bonferroni's test. n = 5 rats for each of the MA experimental groups and n = 4 rats for the control group. ...View More

Nascimento, D., Pozza, D. H., Castro-Lopes, J. M., & Neto, F. L. (2011). Neuronal injury marker ATF-3 is induced in primary afferent neurons of monoarthritic rats. Neurosignals, 19(4), 210-221.

Mouse Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (L32A4) in Immunoblot

Immunoblot analysis of pThr308-Akt and Akt in LV of WT-SED, WT-ET, KO-SED and KO-ET female mice. ...View More

Støle, T. P., Lunde, M., Shen, X., Martinsen, M., Lunde, P. K., Li, J., ... & Carlson, C. R. (2022). The female syndecan-4−/− heart has smaller cardiomyocytes, augmented insulin/pSer473-Akt/pSer9-GSK-3β signaling, and lowered SCOP, pThr308-Akt/Akt and GLUT4 levels. Frontiers in Cell and Developmental Biology, 10, 908126.

Mouse Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (L32A4) in WB

Akt threonine 308 phosphorylation in normoxic and hypoxic NTC and mimic 193a cells was assessed by Western analyses and normalized to total Akt. ...View More

Fuhrmann, D. C., & Brüne, B. (2022). miR-193a-3p increases glycolysis under hypoxia by facilitating akt phosphorylation and PFKFB3 activation in human macrophages. Cellular and Molecular Life Sciences, 79(2), 89.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-443454

Application

Immunogen

Synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

AKT1/AKT2/AKT3

Introduction

AKT is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration.

Alternative Names

AKT 1; AKT2; AKT3

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated S473, S474, S472) Recombinant Antibody (V2-443451) (CAT#: PTM-CBMAB-0027YC)

Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (V2-443453) (CAT#: PTM-CBMAB-0029YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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