Sign in or Register   Sign in or Register
  |  

Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated S473, S474, S472) Recombinant Antibody (V2-443451) (PTM-CBMAB-0027YC)

Provided herein is a Rabbit monoclonal antibody against AKT1 (Ser473)/AKT2 (Ser474)/AKT3 (Ser472). The antibody can be used for immunoassay techniques, such as FC.
See all AKT1/AKT2/AKT3 antibodies
Published Data
Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated S473, S474, S472) Recombinant Antibody (193H12) in Immunoblot
Immunoblot analysis of p/tAKT1, p/tFOXO1, FOXP3, IDO1, BIM, CXCR4 and SDF1 in gingival tissue of CP compared with healthy control. ...View More
Arjunan, P., Meghil, M. M., Pi, W., Xu, J., Lang, L., El-Awady, A., ... & Cutler, C. W. (2018). Oral pathobiont activates anti-apoptotic pathway, promoting both immune suppression and oncogenic cell proliferation. Scientific reports, 8(1), 16607.
Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated S473, S474, S472) Recombinant Antibody (193H12) in FC
SupT1 cells were stimulated with 100 ng/ml CXCL12 in the presence of peptides for 2 min. Afterward, the reaction was stopped by adding 2% PFA and shifting the cells to 4 °C. Cells were then permeabilized and subsequently stained with antibodies against pAkt for analysis in flow cytometry. Shown are data derived from three individual experiments ± SEM. ...View More
Sokkar, P., Harms, M., Stürzel, C., Gilg, A., Kizilsavas, G., Raasholm, M., ... & Sanchez-Garcia, E. (2021). Computational modeling and experimental validation of the EPI-X4/CXCR4 complex allows rational design of small peptide antagonists. Communications Biology, 4(1), 1113.
Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated S473, S474, S472) Recombinant Antibody (193H12) in IF
Confocal microscopy of p-AKT (S473) (red) in SW480 and HCT116 cells transfected with FLAG-tagged BCL-3 (green) or control plasmid. Increased AKT phosphorylation was detected in HCT116 cells overexpressing BCL-3 as well as neighbouring cells with low BCL-3 (arrows). Cell nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue). ...View More
Urban, B. C., Collard, T. J., Eagle, C. J., Southern, S. L., Greenhough, A., Hamdollah-Zadeh, M., ... & Williams, A. C. (2016). BCL-3 expression promotes colorectal tumorigenesis through activation of AKT signalling. Gut, 65(7), 1151-1164.
Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated S473, S474, S472) Recombinant Antibody (193H12) in IHC
IHC staining of FLAG, pMET, pAkt, and Ki67 in transplanted tumors. Representative photographs for each antibody and each group are shown. Scale bars, 50 μm. ...View More
Cheng, F., Zhang, P., Xiao, Q., Li, Y., Dong, M., Wang, H., ... & Guo, D. (2019). The prognostic and therapeutic potential of LRIG3 and soluble LRIG3 in glioblastoma. Frontiers in Oncology, 9, 447.
Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated S473, S474, S472) Recombinant Antibody (193H12) in IP
We constructed the H-RasG12V expression vectors to preferentially activate the Ras pathways. The levels of P-ERK1/2, P-AKT and RalA-GTP were detected with specific antibodies to examine the activities of corresponding pathways. ...View More
Liu, Y., Wang, S. Q., Long, Y. H., Chen, S., Li, Y. F., & Zhang, J. H. (2016). KRASG12 mutant induces the release of the WSTF/NRG3 complex, and contributes to an oncogenic paracrine signaling pathway. Oncotarget, 7(33), 53153.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
V2-443451
Antibody Isotype
IgG
Application
IHC-F, WB, IP, IF

Basic Information

Immunogen
Synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:200
IF(ICC)1:200
FC1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AKT1/AKT2/AKT3
Introduction
AKT is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration.
Alternative Names
AKT 1; AKT2; AKT3
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated S473, S474, S472) Recombinant Antibody (V2-443451)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare