Mouse Anti-CAPZB Recombinant Antibody (CBYY-C0944) (CBMAB-C2381-YY)

Name: Mouse Anti-CAPZB Recombinant Antibody (CBYY-C0944)
SKU: CBMAB-C2381-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CAPZB Recombinant Antibody (CBYY-C0944) (CBMAB-C2381-YY) in WB

Western blot of wild type (WT) G1E cell lysates after retroviral infection with control shRNA (shControl) or shRNA targeting Capzb mRNA (shCapzb2) stained with anti-sterile alpha motif domain 14 (Samd14), anti-Capzα1, anti-Capzβ, or anti-Hsc70 antibodies. ...View More

Ray, S., Chee, L., Zhou, Y., Schaefer, M. A., Naldrett, M. J., Alvarez, S., ... & Hewitt, K. J. (2022). Functional requirements for a Samd14-capping protein complex in stress erythropoiesis. Elife, 11, e76497.

Mouse Anti-CAPZB Recombinant Antibody (CBYY-C0944) (CBMAB-C2381-YY) in WB

Western blot of WT G1E cell lysates following pulldown with anti-Samd14 or anti-rabbit IgG control antibody. Input corresponds to 5% of immunoprecipitation (IP) lysate. ...View More

Mouse Anti-CAPZB Recombinant Antibody (CBYY-C0944) (CBMAB-C2381-YY) in IF

The distribution of phalloidin stained F-actin (red) and of CP visualized by HA-Tag antibody labelling (green) in fixed cells is shown: In the top cells overexpressed CP after induction with 1 µg/ml Doxycyclin (CP Dox, N = 21). In the middle cells were not overexpressing CP but treated with 1 µg/ml Doxycyclin (no CP Dox, N = 10) and in bottom cells contained the expression plasmids but were not treated with Doxycyclin (CP no Dox, N = 21). ...View More

Dürre, K., Keber, F. C., Bleicher, P., Brauns, F., Cyron, C. J., Faix, J., & Bausch, A. R. (2018). Capping protein-controlled actin polymerization shapes lipid membranes. Nature communications, 9(1), 1630.

Mouse Anti-CAPZB Recombinant Antibody (CBYY-C0944) (CBMAB-C2381-YY) in IF

Confocal images of the equatorial vesicle's plane showed the interactions of the contractile actin network (red hot) with the membrane (cyan). At 20 nM CP the network detached from the membrane and was pulled towards the bulk of the vesicle. At 60 nM CP membrane invaginations (insets) emerged from the membrane. At 120 nM CP membrane invaginations (left inset) and fission events (right insets) could be observed. Scale bars are 20 µm. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C0944

Application

IP, WB

Immunogen

Amino acids 162-267 of CapZ-β of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Chicken

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

capping protein (actin filament) muscle Z-line, beta

Entrez Gene ID

832

UniProt ID

P47756

Alternative Names

Caspase 3; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Apopain; CASP-3;

Function

F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization.

Biological Process

Actin cytoskeleton organization Source: InterPro
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Barbed-end actin filament capping Source: GO_Central
Blood coagulation Source: Reactome
Cell morphogenesis Source: GO_Central
Cytoskeleton organization Source: UniProtKB
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Negative regulation of filopodium assembly Source: GO_Central
Regulation of cell morphogenesis Source: UniProtKB
Regulation of lamellipodium assembly Source: GO_Central

Cellular Location

Cytoskeleton; Sarcomere

Radloff, A., Breckpot, J., Hoff, K., Kramer, H. H., Panáková, D., Mercks, A., ... & Hitz, M. P. (2018). CAPZB: A Potential Novel Key Player in Cardiac Development and Congenital Heart Defects. The Thoracic and Cardiovascular Surgeon, 66(S 02), DGPK-V243.

Xiong, Z., Zhang, H., Huang, B., Liu, Q., Wang, Y., Shi, D., & Li, X. (2018). Expression pattern of prohibitin, capping actin protein of muscle Z-line beta subunit and tektin-2 gene in Murrah buffalo sperm and its relationship with sperm motility. Asian-Australasian journal of animal sciences, 31(11), 1729.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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