Mouse Anti-ADIPOR1 Recombinant Antibody (V2-179982) (CBMAB-A1368-YC)

Name: Mouse Anti-ADIPOR1 Recombinant Antibody (V2-179982)
SKU: CBMAB-A1368-YC
Rating: 5 (1 reviews)

See all ADIPOR1 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-ADIPOR1 Recombinant Antibody (CBYC-A369) (CBMAB-A1368-YC) in WB

HFD regulates cochlear AdipoR1, COX IV, HO-1 and G3bp1; and liver AdipoR1. Cochlear AdipoR1, cytochrome c oxidase subunit IV (COX IV) heme oxygenase-1 (HO-1; also known as heat shock protein 32, HSP 32), and a marker of stress granule (G3bp1) levels were measured in the whole cochlear lysates by Western blot analysis (a) and quantified (b-e). Cochlear AdipoR1 and HO-1 presented main effects of sex and diet while COX IV and G3bp1 displayed a main effect of diet. f Liver AdipoR1 protein levels were measured by Western blotting (a) and quantified (g). Female AdipoR1 levels were regulated by diet, but no changes were seen in male AdipoR1 in the liver. Different letters indicate significant differences between treatments. Two-way ANOVA with Tukey's multiple comparisons test. p < 0.05. amain effect of sex; bmain effect of diet; cinteraction effect. ...View More

Kim, S. J., Gajbhiye, A., Lyu, A. R., Kim, T. H., Shin, S. A., Kwon, H. C., ... & Park, M. J. (2023). Sex differences in hearing impairment due to diet-induced obesity in CBA/Ca mice. Biology of Sex Differences, 14(1), 10.

Mouse Anti-ADIPOR1 Recombinant Antibody (CBYC-A369) (CBMAB-A1368-YC) in FC

AdipoR1 is involved in IR-induced ferroptosis in HCC cells. (a) MHCC-97H cells were pretreated with different inhibitors of cell death 3-MA, ZVAD, Rapa, and Fer-1 and irradiated. The cell viability was detected by CCK-8 kit. (b-e) After treatment with 10 Gy for 48 h, lipid peroxidation was assessed by flow cytometry using C11-BODIPY in AdipoR1 knockdown MHCC-97H (b and c) and HepG2 (d and e) cells, respectively. (f and g) qRT-PCR showed the mRNA level of PTGS2 in HCC cells after radiation or AdipoR1 knockdown. Data is presented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. ...View More

Feng, H., Liu, Y., Gan, Y., Li, M., Liu, R., Liang, Z., ... & Ma, S. (2022). AdipoR1 Regulates Ionizing Radiation-Induced Ferroptosis in HCC cells through Nrf2/xCT Pathway. Oxidative Medicine and Cellular Longevity, 2022.

Mouse Anti-ADIPOR1 Recombinant Antibody (CBYC-A369) (CBMAB-A1368-YC) in IF

Immunofluorescence results of the expression of AdipoR 1 protein in hippocampus. ...View More

Yan, W., Gao, S., Zhang, Q., Qi, J., Liu, G., Teng, Y., ... & Ji, B. (2022). AdipoRon Inhibits Neuroinflammation Induced by Deep Hypothermic Circulatory Arrest Involving the AMPK/NF-κB Pathway in Rats. Pharmaceutics, 14(11), 2467.

Mouse Anti-ADIPOR1 Recombinant Antibody (CBYC-A369) (CBMAB-A1368-YC) in WB

Mut-Reg1cp promoted insulin resistance by inhibiting AdipoR1 translation.
A Mut-Reg1cp retrieved PTBP1, as detected by immunoblotting. B, C Representative pictures (B) and quantitative measurements (C) of western blot analysis of PTBP1 and ADIPOR1 level in C2C12 myocytes transfected with Mut-Reg1cp or control plasmids with or without si-Ptbp1 treatment. D, E Representative pictures (D) and quantitative measurements (E) of western blot analysis of ADIPOR1 level in C2C12 myocytes treated with exosomes contained Mut-Reg1cp or not. F, G Representative pictures (F) and quantitative measurements (G) of western blot analysis of ADIPOR1 level in Hepa1-6 hepatocytes treated with exosomes contained Mut-Reg1cp or not. H, I Representative pictures (H) and quantitative measurements (I) of western blot analysis of ADIPOR1 level in muscle of Reg1cp-mutRIP+ mice and Rosa-Reg1cp-mut controls. J, K Representative pictures (J) and quantitative measurements (K) of western blot analysis of ADIPOR1 level in liver of Reg1cp-mutRIP+ mice and Rosa-Reg1cp-mut controls. L Semi-quantitative PCR showed the expression level of Reg1cp in exosomes isolated from human serum. M, N Representative pictures (M) and quantitative measurements (N) of western blot analysis of ADIPOR1, p-AMPK and t-AMPK level in HepG2 hepatocytes treated with exosomes isolated from human serum. A and L were representative of two independent experiments. In B-K, M, N, n = 6 in each group from two independent experiments. Data shown as mean ± SD. *P < 0.05; **P < 0.01; NS no significance; Student's t test for E, G, I, K, N. One-way ANOVA for C. ...View More

Guo, W. H., Guo, Q., Liu, Y. L., Yan, D. D., Jin, L., Zhang, R., ... & Yang, M. (2022). Mutated lncRNA increase the risk of type 2 diabetes by promoting β cell dysfunction and insulin resistance. Cell Death & Disease, 13(10), 904.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179982

Application

WB, IP, IF, ELISA

Immunogen

Specific for an epitope mapping between amino acids 16-41 at the N-terminus of AdipoR1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.2

Buffer

PBS, 0.1% gelatin.

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

adiponectin receptor 1

Introduction

ADIPOR1 is a protein which acts as a receptor for adiponectin, a hormone secreted by adipocytes which regulates fatty acid catabolism and glucose levels. Binding of adiponectin to the encoded protein results in activation of an AMP-activated kinase signal

Entrez Gene ID

51094

UniProt ID

Q96A54

Alternative Names

Adiponectin Receptor 1; Progestin And AdipoQ Receptor Family Member 1; Progestin And AdipoQ Receptor Family Member I; TESBP1A; PAQR1; Adiponectin Receptor Protein 1; ACDCR1; CGI-45; CGI45;

Function

Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin (By similarity).

Biological Process

Adiponectin-activated signaling pathway
Fatty acid oxidation
Glucose homeostasis
Hormone-mediated signaling pathway
Leptin-mediated signaling pathway
Negative regulation of cell growth
Negative regulation of epithelial cell migration
Negative regulation of epithelial to mesenchymal transition
Negative regulation of NIK/NF-kappaB signaling
Negative regulation of receptor signaling pathway via JAK-STAT
Positive regulation of cold-induced thermogenesis
Positive regulation of insulin receptor signaling pathway
Positive regulation of receptor signaling pathway via JAK-STAT
Regulation of fatty acid biosynthetic process
Regulation of glucose metabolic process
Regulation of lipid metabolic process

Cellular Location

Cell membrane. Localized to the cell membrane and intracellular organelles.

Topology

Cytoplasmic: 1-136 aa
Helical: 137-157 aa
Extracellular: 158-170 aa
Helical: 171-191 aa
Cytoplasmic: 192-203 aa
Helical: 204-224 aa
Extracellular: 225-234 aa
Helical: 235-255 aa
Cytoplasmic: 256-264 aa
Helical: 265-285 aa
Extracellular: 286-298 aa
Helical: 299-319 aa
Cytoplasmic: 320-337 aa
Helical: 338-358 aa
Extracellular: 359-375 aa

Chen, Y. J., Lin, C. W., Peng, Y. J., Huang, C. W., Chien, Y. S., Huang, T. H., ... & Ding, S. T. (2021). Overexpression of Adiponectin Receptor 1 Inhibits Brown and Beige Adipose Tissue Activity in Mice. International journal of molecular sciences, 22(2), 906.

Cicekdal, M. B., Kazan, B. T., Tuna, B. G., Ozorhan, U., Ekici, I. D., Zhu, F., ... & Yilmaz, B. (2021). Effects of two types of energy restriction on methylation levels of adiponectin receptor 1 and leptin receptor overlapping transcript in a mouse mammary tumour virus-transforming growth factor-α breast cancer mouse model. British Journal of Nutrition, 125(1), 1-9.

Ramos-Ramírez, P., Malmhäll, C., Johansson, K., Adner, M., Lötvall, J., & Bossios, A. (2020). Lung Regulatory T Cells Express Adiponectin Receptor 1: Modulation by Obesity and Airway Allergic Inflammation. International Journal of Molecular Sciences, 21(23), 8990.

Tanabe, H., Fujii, Y., Okada-Iwabu, M., Iwabu, M., Kano, K., Kawana, H., ... & Yokoyama, S. (2020). Human adiponectin receptor AdipoR1 assumes closed and open structures. Communications biology, 3(1), 1-11.

Pal, S., Porwal, K., Rajak, S., Sinha, R. A., & Chattopadhyay, N. (2020). Selective dietary polyphenols induce differentiation of human osteoblasts by adiponectin receptor 1-mediated reprogramming of mitochondrial energy metabolism. Biomedicine & Pharmacotherapy, 127, 110207.

Pepin, M. E., Koentges, C., Pfeil, K., Gollmer, J., Kersting, S., Wiese, S., ... & Bugger, H. (2019). Dysregulation of the mitochondrial proteome occurs in mice lacking adiponectin receptor 1. Frontiers in endocrinology, 10, 872.

Zhao, L., Chen, S., Sherchan, P., Ding, Y., Zhao, W., Guo, Z., ... & Zhang, J. H. (2018). Recombinant CTRP9 administration attenuates neuroinflammation via activating adiponectin receptor 1 after intracerebral hemorrhage in mice. Journal of neuroinflammation, 15(1), 1-14.

Kim, S., Lee, Y., Kim, J. W., Son, Y. J., Ma, M. J., Um, J. H., ... & Kim, B. B. (2018). Discovery of a novel potent peptide agonist to adiponectin receptor 1. PLoS One, 13(6), e0199256.

Peng, Y. J., Shen, T. L., Chen, Y. S., Mersmann, H. J., Liu, B. H., & Ding, S. T. (2018). Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. Journal of biomedical science, 25(1), 1-13.

Kim, M. W., Bin Abid, N., Jo, M. H., Jo, M. G., Yoon, G. H., & Kim, M. O. (2017). Suppression of adiponectin receptor 1 promotes memory dysfunction and Alzheimer’s disease-like pathologies. Scientific reports, 7(1), 1-10.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ADIPOR1 Recombinant Antibody (V2-179982)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ADIPOR1 Recombinant Antibody (V2-179981) (CAT#: CBMAB-A1367-YC)

Mouse Anti-ADIPOR1 Recombinant Antibody (V2-179980) (CAT#: CBMAB-A1366-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry