Rat Anti-CD63 Recombinant Antibody (7G4.2E8) (CBMAB-C8725-LY)

Name: Rat Anti-CD63 Recombinant Antibody (7G4.2E8)
SKU: CBMAB-C8725-LY
Rating: 5 (1 reviews)

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Published Data

Rat Anti-CD63 Recombinant Antibody (7G4.2E8) (CBMAB-C8725-LY) in WB

Western blot analysis of isolated exosomes. The presence of canonical exosome proteins and the HSP70 decreasing indicate a pure exosome preparation. ...View More

Chen, Y. Y., Lee, Y. H., Wang, B. J., Chen, R. J., & Wang, Y. J. (2022). Skin damage induced by zinc oxide nanoparticles combined with UVB is mediated by activating cell pyroptosis via the NLRP3 inflammasome-autophagy-exosomal pathway. Particle and fibre toxicology, 19, 1-22. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

7G4.2E8

Application

WB, FC

Immunogen

Partial recombinant mouse CD63 protein (between amino acids 100-250).

Host Species

Rat

Specificity

Mouse, Human

Antibody Isotype

IgG2a, λ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
FC	2 μg/10^6 cells
WB	2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD63 Molecule

Introduction

CD63 (CD63 Molecule) is a Protein Coding gene. Diseases associated with CD63 include Hermansky-Pudlak Syndrome and Melanoma. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Innate Immune System.

An important paralog of this gene is TSPAN33.

Entrez Gene ID

Human	967
Mouse	12512

UniProt ID

Human	P08962
Mouse	P41731

Function

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

Biological Process

Cell-matrix adhesion Source: UniProtKB
Cell migration Source: UniProtKB
Cellular protein localization Source: MGI
Endosome to melanosome transport Source: UniProtKB
Neutrophil degranulation Source: Reactome
Pigment granule maturation Source: UniProtKB
Platelet degranulation Source: Reactome
Positive regulation of integrin-mediated signaling pathway Source: UniProtKB
Positive regulation of receptor internalization Source: UniProtKB
Protein transport Source: UniProtKB-KW
Regulation of potassium ion transmembrane transport Source: MGI
Regulation of vascular endothelial growth factor signaling pathway Source: UniProtKB

Cellular Location

Lysosome membrane; Cell membrane; Extracellular exosome; Late endosome membrane; Multivesicular body; Melanosome; Cell surface. Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903).

Topology

Cytoplasmic: 2-11
Helical: 12-32
Extracellular: 33-51
Helical: 52-72
Cytoplasmic: 73-81
Helical: 82-102
Extracellular: 103-203
Helical: 204-224
Cytoplasmic: 225-238

PTM

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).

Fan, Z. H., Xu, Y., Luo, W., He, X. C., Zheng, T. T., Zhang, J. J., ... & Lee, X. Z. (2021). Molecular cloning and characterization of CD63 in common carp infected with koi herpesvirus. Developmental & Comparative Immunology, 121, 104102.

Mathieu, M., Névo, N., Jouve, M., Valenzuela, J. I., Maurin, M., Verweij, F. J., ... & Théry, C. (2021). Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nature Communications, 12(1), 1-18.

Mathieu, M., Névo, N., Jouve, M., Valenzuela, J. I., Maurin, M., Verweij, F., ... & Théry, C. (2020). Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking and synchronized extracellular vesicle release of CD9 and CD63. bioRxiv.

Dogrammatzis, C., Deschamps, T., & Kalamvoki, M. (2019). Biogenesis of extracellular vesicles during herpes simplex virus 1 infection: role of the CD63 tetraspanin. Journal of virology, 93(2), e01850-18.

Wang, L., Giri, B. R., Chen, Y., Xia, T., Liu, J., Li, H., ... & Cheng, G. (2018). Molecular characterization, expression profile, and preliminary evaluation of diagnostic potential of CD63 in Schistosoma japonicum. Parasitology research, 117(11), 3625-3631.

Hurwitz, S. N., Cheerathodi, M. R., Nkosi, D., York, S. B., & Meckes Jr, D. G. (2018). Tetraspanin CD63 bridges autophagic and endosomal processes to regulate exosomal secretion and intracellular signaling of Epstein-Barr virus LMP1. Journal of virology, 92(5), e01969-17.

Priyathilaka, T. T., Bathige, S. D. N. K., Herath, H. M. L. P. B., Lee, S., & Lee, J. (2017). Molecular identification of disk abalone (Haliotis discus discus) tetraspanin 33 and CD63: Insights into potent players in the disk abalone host defense system. Fish & shellfish immunology, 69, 173-184.

Raaben, M., Jae, L. T., Herbert, A. S., Kuehne, A. I., Stubbs, S. H., Chou, Y. Y., ... & Whelan, S. P. (2017). NRP2 and CD63 are host factors for Lujo virus cell entry. Cell host & microbe, 22(5), 688-696.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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