CD63 Antibodies

Structure of CD63

CD63 is a transmembrane protein with a molecular weight of approximately 25-50 kDa, and its specific molecular weight varies depending on the degree of glycosylation modification. This protein belongs to the tetraspanin superfamily and has a highly conserved structural core in different species.

The CD63 protein is composed of 239 amino acids, forming four highly conserved transmembrane domains (TM1-TM4), two extracellular loops (EC1, EC2), and the intracellular N-terminus and C-terminus. Among them, the larger second extracellular loop (EC2) contains a characteristic "CCG" motif and conserved disulfide bonds, which are involved in protein-protein interactions. This protein regulates membrane protein transport and cellular signal transduction by forming the tetraspanin web. Its intracellular distribution is mainly in the endosome/lysosomal system and is widely used as a marker of exosomes.

Fig. 1 Schematic representation of the probable structure of CD63.¹

Key structural properties of CD63:

• Four transmembrane domain structure formed four typical polymers conformation
• Extracellular the second (EC2) including conservative CCG die bodies and disulfide bond
• Intracellular C tail including lysosomal targeting signal

Functions of CD63

The main function of the CD63 protein is to participate in intracellular transport and exosome formation. However, it also involves a variety of cellular processes, including cell signaling and membrane protein organization.

CD63 interacts specifically with various ligands and membrane proteins through its extracellular domain to form functional complexes, thereby widely participating in cell membrane compartmentalization and transmembrane signal transduction events.

Applications of CD63 and CD63 Antibody in Literature

1. Wang, Xinyue, et al. "A novel rabbit anti-myoglobin monoclonal antibody's potential application in rhabdomyolysis associated acute kidney injury." International Journal of Molecular Sciences 24.9 (2023): 7822. https://doi.org/10.3390/v13040675

The article indicates that CD63 is a quadruple transmembrane protein that participates in endocytic transport and extracellular vesicle cargo sorting. Research has found that LMP1 can alter the interaction group of CD63, shifting it from transport function to metabolic and translation processes, and may form complexes with mTOR and others to regulate signal transduction.

2. Odaka, Haruki, et al. "CD63-positive extracellular vesicles are potential diagnostic biomarkers of pancreatic ductal adenocarcinoma." BMC gastroenterology 22.1 (2022): 153. https://doi.org/10.1186/s12876-022-02228-7

The article indicates that the level of CD63+ extracellular vesicles in the serum of patients with pancreatic ductal adenocarcinoma (PDAC) is significantly elevated, demonstrating excellent diagnostic performance (AUC: 0.846), especially when combined with CA19-9 in the early stage (AUC: 0.903), and the level decreases after surgery, showing its potential as a biomarker for PDAC.

3. Danquah, Bright D., et al. "Mass Spectrometric analysis of antibody—Epitope peptide complex dissociation: Theoretical concept and practical procedure of binding strength characterization." Molecules 25.20 (2020): 4776. https://doi.org/10.1038/s41598-021-99777-w

The article indicates that the level of CD63 in plasma exosomes is significantly elevated in patients with sepsis, positively correlated with the SOFA score. Moreover, a high level of CD63 (>126 µg/mL) can predict 28-day in-hospital mortality and 90-day survival, suggesting its potential as a biomarker for evaluating the severity and prognosis of sepsis.

4. Justo, Beatriz Laís, and Miriam Galvonas Jasiulionis. "Characteristics of TIMP1, CD63, and β1-integrin and the functional impact of their interaction in cancer." International journal of molecular sciences 22.17 (2021): 9319. https://doi.org/10.3390/ijms22179319

The article indicates that TIMP-1 can form a membrane complex with CD63 and β 1-integrin. This complex not only inhibits the activity of metalloproteinases but also participates in regulating cell growth and survival signaling pathways. Moreover, it is highly expressed in various tumors and may be associated with a poor prognosis. Its assembly and function are regulated by N-glycosylation.

5. Fan, Yé, et al. "Differential proteomics argues against a general role for CD9, CD81 or CD63 in the sorting of proteins into extracellular vesicles." Journal of Extracellular Vesicles 12.8 (2023): 12352. https://doi.org/10.1002/jev2.12352

The article indicates that CD63, CD9 and CD81 are the main marker proteins of extracellular vesicles (EVs), but in MCF7 cells, their influence on the protein composition of EVs is limited. The co-deletion of CD9 and CD81 will significantly reduce the contents of their ligands CD9P-1 and EWI-2 in EVs. These four-transmembrane proteins are more involved in regulating the expression and transport of related proteins rather than directly determining the composition of EVs.

Creative Biolabs: CD63 Antibodies for Research

Creative Biolabs specializes in the production of high-quality CD63 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom CD63 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our CD63 antibodies, custom preparations, or technical support, contact us at email.