Mouse Anti-CCDC25 Recombinant Antibody (CBLC132-LY) (CBMAB-C9786-LY)

Name: Mouse Anti-CCDC25 Recombinant Antibody (CBLC132-LY)
SKU: CBMAB-C9786-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCDC25 Recombinant Antibody (CBLC132-LY) (CBMAB-C9786-LY) in IHC

IHC results of CCDC25 in controls and GCMs. ...View More

Hu, Z., Hua, X., Mo, X., Chang, Y., Chen, X., Xu, Z., ... & Song, J. (2023). Inhibition of NETosis via PAD4 alleviated inflammation in giant cell myocarditis. Iscience, 26(7).

Mouse Anti-CCDC25 Recombinant Antibody (CBLC132-LY) (CBMAB-C9786-LY) in ELISA

The expression of NET-receptor CCDC25 is not up-regulated in human aortic endothelial cells (HAoEC) treated with NETs of essential hypertension (EH). (A) Fold expression of CCDC25 mRNA in control neutrophils stimulated with NETs released from control neutrophils treated with plasma from EH patients (EH-NETs). RPL13A was used to normalize gene expression. (B) Surface expression of CCDC25 assessed by In-Cell ELISA in HAoEC treated with EH-NETs. For (A)-(B): Data are from four independent experiments (mean ± SD). All conditions were compared to untreated (statistically significant: p< 0.05, n.s.: not significant), Wilcoxon test for paired samples. ...View More

Chrysanthopoulou, A., Gkaliagkousi, E., Lazaridis, A., Arelaki, S., Pateinakis, P., Ntinopoulou, M., ... & Skendros, P. (2021). Angiotensin II triggers release of neutrophil extracellular traps, linking thromboinflammation with essential hypertension. JCI insight, 6(18).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLC132-LY

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 61-137 mapping within an internal region of CCDC25 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Coiled-Coil Domain Containing 25

Introduction

CCDC25 (Coiled-Coil Domain Containing 25) is a Protein Coding gene.

Entrez Gene ID

Human	55246
Mouse	67179
Rat	361059

UniProt ID

Human	Q86WR0
Mouse	Q78PG9
Rat	D4AAU6

Function

Transmembrane receptor that senses neutrophil extracellular traps (NETs) and triggers the ILK-PARVB pathway to enhance cell motility (PubMed:32528174).
NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:32528174).
Formation of NETs is also associated with cancer metastasis, NET-DNA acting as a chemotactic factor to attract cancer cells (PubMed:32528174).
Specifically binds NETs on its extracellular region, in particular the 8-OHdG-enriched DNA present in NETs, and recruits ILK, initiating the ILK-PARVB cascade to induce cytoskeleton rearrangement and directional migration of cells (PubMed:32528174).
In the context of cancer, promotes cancer metastasis by sensing NETs and promoting migration of tumor cells (PubMed:32528174).

Biological Process

Positive regulation of cell motility Source: UniProtKB

Cellular Location

Cell membrane; Endomembrane system. Localizes to cytoplasmic membrane in tumor cells.

Topology

Extracellular: 1-66
Helical: 67-83
Cytoplasmic: 84-208

Siriphak, S., Chanakankun, R., Proungvitaya, T., Roytrakul, S., Tummanatsakun, D., Seubwai, W., ... & Proungvitaya, S. (2021). Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis. Molecules, 26(11), 3105.

Chanakankun, R., Proungvitaya, T., Chua‑On, D., Limpaiboon, T., Roytrakul, S., Jusakul, A., ... & Proungvitaya, S. (2020). Serum coiled‑coil domain containing 25 protein as a potential screening/diagnostic biomarker for cholangiocarcinoma. Oncology letters, 19(1), 930-942.

Liu, R., Zhao, E., Wang, F., & Cui, H. (2020). CCDC25: precise navigator for neutrophil extracellular traps on the prometastatic road. Signal Transduction and Targeted Therapy, 5(1), 1-2.

Dickson, I. (2020). NETs promote liver metastasis via CCDC25. Nature Reviews Gastroenterology & Hepatology, 17(8), 451-451.

Yang, L., Liu, Q., Zhang, X., Liu, X., Zhou, B., Chen, J., ... & Song, E. (2020). DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25. Nature, 583(7814), 133-138.

Proungvitaya, S., Klinthong, W., Proungvitaya, T., Limpaiboon, T., Jearanaikoon, P., Roytrakul, S., ... & Wongkham, S. (2017). High expression of CCDC25 in cholangiocarcinoma tissue samples. Oncology letters, 14(2), 2566-2572.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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