Mouse Anti-CCDC25 Recombinant Antibody (CBLC132-LY) (CBMAB-C9786-LY)

The product is antibody recognizes CCDC25. The antibody CBLC132-LY immunoassay techniques such as: WB, IP, IF, ELISA.
See all CCDC25 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse, Rat, Human

Clone

CBLC132-LY

Antibody Isotype

IgG

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Coiled-Coil Domain Containing 25

Introduction

CCDC25 (Coiled-Coil Domain Containing 25) is a Protein Coding gene.

Entrez Gene ID

Human	55246
Mouse	67179
Rat	361059

UniProt ID

Human	Q86WR0
Mouse	Q78PG9
Rat	D4AAU6

Function

Transmembrane receptor that senses neutrophil extracellular traps (NETs) and triggers the ILK-PARVB pathway to enhance cell motility (PubMed:32528174).
NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:32528174).
Formation of NETs is also associated with cancer metastasis, NET-DNA acting as a chemotactic factor to attract cancer cells (PubMed:32528174).
Specifically binds NETs on its extracellular region, in particular the 8-OHdG-enriched DNA present in NETs, and recruits ILK, initiating the ILK-PARVB cascade to induce cytoskeleton rearrangement and directional migration of cells (PubMed:32528174).
In the context of cancer, promotes cancer metastasis by sensing NETs and promoting migration of tumor cells (PubMed:32528174).

Biological Process

Positive regulation of cell motility Source: UniProtKB

Cellular Location

Cell membrane; Endomembrane system. Localizes to cytoplasmic membrane in tumor cells.

Topology

Extracellular: 1-66
Helical: 67-83
Cytoplasmic: 84-208

References

Siriphak, S., Chanakankun, R., Proungvitaya, T., Roytrakul, S., Tummanatsakun, D., Seubwai, W., ... & Proungvitaya, S. (2021). Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis. Molecules, 26(11), 3105.

Chanakankun, R., Proungvitaya, T., Chua‑On, D., Limpaiboon, T., Roytrakul, S., Jusakul, A., ... & Proungvitaya, S. (2020). Serum coiled‑coil domain containing 25 protein as a potential screening/diagnostic biomarker for cholangiocarcinoma. Oncology letters, 19(1), 930-942.

Liu, R., Zhao, E., Wang, F., & Cui, H. (2020). CCDC25: precise navigator for neutrophil extracellular traps on the prometastatic road. Signal Transduction and Targeted Therapy, 5(1), 1-2.

Dickson, I. (2020). NETs promote liver metastasis via CCDC25. Nature Reviews Gastroenterology & Hepatology, 17(8), 451-451.

Yang, L., Liu, Q., Zhang, X., Liu, X., Zhou, B., Chen, J., ... & Song, E. (2020). DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25. Nature, 583(7814), 133-138.

Proungvitaya, S., Klinthong, W., Proungvitaya, T., Limpaiboon, T., Jearanaikoon, P., Roytrakul, S., ... & Wongkham, S. (2017). High expression of CCDC25 in cholangiocarcinoma tissue samples. Oncology letters, 14(2), 2566-2572.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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