Mouse Anti-CCNH Recombinant Antibody (CBFYC-1054) (CBMAB-C1111-FY)

Name: Mouse Anti-CCNH Recombinant Antibody (CBFYC-1054)
SKU: CBMAB-C1111-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCNH Recombinant Antibody (CBFYC-1054) (CBMAB-C1111-FY) in IF

Pol II CTD kinase nuclear signals in siIws1;Supt6 2-cell embryos. Doubleimmunostaining of Cdk9/Cyclin T1 and Cdk7/Cyclin H reveals that the nuclear signals are unchanged in DKD embryos compared to control embryos. Scale bar: 20 μm. ...View More

Oqani, R. K., Lin, T., Lee, J. E., Kang, J. W., Shin, H. Y., & Il Jin, D. (2019). Iws1 and Spt6 regulate trimethylation of histone H3 on lysine 36 through Akt signaling and are essential for mouse embryonic genome activation. Scientific reports, 9(1), 3831.

Mouse Anti-CCNH Recombinant Antibody (CBFYC-1054) (CBMAB-C1111-FY) in WB

HCMV strain TB40 was used to address the question of strain independence of pUL97-cyclin H interaction. ...View More

Steingruber, M., Kraut, A., Socher, E., Sticht, H., Reichel, A., Stamminger, T., ... & Marschall, M. (2016). Proteomic interaction patterns between human cyclins, the cyclin-dependent kinase ortholog pUL97 and additional cytomegalovirus proteins. Viruses, 8(8), 219.

Mouse Anti-CCNH Recombinant Antibody (CBFYC-1054) (CBMAB-C1111-FY) in IP

CoIP analyses with recombinantly expressed pUL53-Flag served as a negative control and the self-interaction of pUL97 as the positive control. ...View More

Graf, L., Webel, R., Wagner, S., Hamilton, S. T., Rawlinson, W. D., Sticht, H., & Marschall, M. (2013). The cyclin-dependent kinase ortholog pUL97 of human cytomegalovirus interacts with cyclins. Viruses, 5(12), 3213-3230.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1054

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Full length cyclin H of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

N-terminus

More Infomation

Target

Full Name

Cyclin H

Introduction

CCNH (Cyclin H) is a Protein Coding gene. Diseases associated with CCNH include Breast Cancer and Fructose Intolerance, Hereditary. Among its related pathways are RNA Polymerase II Transcription Initiation And Promoter Clearance and Formation of HIV elongation complex in the absence of HIV Tat. Gene Ontology (GO) annotations related to this gene include protein kinase binding and DNA-dependent ATPase activity.

Entrez Gene ID

902

UniProt ID

P51946

Alternative Names

Cyclin H; MO15-Associated Protein; Cyclin-Dependent Kinase-Activating Kinase Complex Subunit; CDK-Activating Kinase Complex Subunit; CAK Complex Subunit; P34

Function

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Biological Process

7-methylguanosine mRNA capping Source: Reactome
G1/S transition of mitotic cell cycle Source: Reactome
G2/M transition of mitotic cell cycle Source: Reactome
Nucleotide-excision repair, preincision complex assembly Source: Reactome
Phosphorylation of RNA polymerase II C-terminal domain Source: GO_Central
Protein stabilization Source: CAFA
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central
Termination of RNA polymerase I transcription Source: Reactome
Transcription by RNA polymerase II Source: UniProtKB
Transcription-coupled nucleotide-excision repair Source: Reactome
Transcription elongation from RNA polymerase II promoter Source: Reactome
Transcription initiation from RNA polymerase II promoter Source: ARUK-UCL
Transcription initiation from RNA polymerase I promoter Source: Reactome

Cellular Location

Nucleus

Mao, L., Ling, X., & Chen, J. (2021). Cyclin H Regulates Lung Cancer Progression as a Carcinoma Inducer. Computational and Mathematical Methods in Medicine, 2021.

Mamoor, S. (2020). Cyclin H is differentially expressed in the primary tumors of breast cancer patients treated with trastuzumab.

Mamoor, S. (2020). Six homeobox 6 (SIX6) is differentially expressed in the primary tumors of breast cancer patients treated with trastuzumab.

Palugulla, S., Devaraju, P., Kayal, S., Narayan, S. K., & Mathaiyan, J. (2018). Genetic polymorphisms in cyclin H gene are associated with oxaliplatin-induced acute peripheral neuropathy in South Indian digestive tract cancer patients. Cancer chemotherapy and pharmacology, 82(3), 421-428.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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