What applications has this antibody been validated for?

Based on typical usage and the positive results obtained, this antibody is effective and validated for use in Western Blot (WB), Immunofluorescence (IF), and Immunohistochemistry (IHC). Refer to the product datasheet for specific recommended dilutions and protocols for each application.

What is the recommended starting dilution for Western Blot?

A good starting point for Western Blot is typically a dilution between 1:1000 and 1:5000. However, optimal dilution may vary depending on your experimental setup, sample type, and detection system. We recommend performing a dilution series to determine the most optimal working dilution for your specific needs. Q3: Is antigen retrieval required for immunohistochemistry (IHC) with this antibody?

Mouse Anti-14-3-3 Pan Recombinant Antibody (V2-9272) (CBMAB-1181-LY)

See all 14-3-3 Pan antibodies

Compare Online Inquiry

Published Data

Mouse Anti-14-3-3 Pan Recombinant Antibody (3F7) (CBMAB-1181-LY) in WB

Western-blot analysis of selected target proteins confirmed TXNL1, CAPZA1, and HDAC2 as significantly differentially expressed proteins (highlighted in bold). TXNL1 and CAPZA1 are significantly lower expressed in aneuploid cell lines whereas HDAC2 significantly higher expressed in aneuploid cell lines (*Fold change calculation for western blot (WB) and two-dimensional gel electrophoresis (2-DE): aneuploid over diploid) ...View More

Gemoll, T., Roblick, U. J., Szymczak, S., Braunschweig, T., Becker, S., Igl, B. W., ... & Habermann, J. K. (2011). HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers. Cellular and Molecular Life Sciences, 68, 3261-3274.

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-9272

Application

IP, WB

Immunogen

Full length protein.

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

14-3-3 Pan

Introduction

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-14-3-3 Pan Recombinant Antibody (V2-9272)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry