Mouse Anti-AMOT Recombinant Antibody (CBYC-A564) (CBMAB-A2552-YC)

Name: Mouse Anti-AMOT Recombinant Antibody (CBYC-A564)
SKU: CBMAB-A2552-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AMOT Recombinant Antibody (CBYC-A564) in FC

E7-induced F-actin alterations interfere with p-YAP/AMOT1 interactions. Quantitative evaluation of F-actin/AMOT1 molecular association by FRET technique, as revealed by flow cytometry analysis restricted to pAmCyan-positive cells. Numbers indicate the percentage of FL3-positive events, obtained in one experiment representative of three. Bar graphs on right show FRET efficiency calculated according to the Riemann's algorithm. Data are reported as mean ± SD from three independent experiments. Inset: representative flow cytometry histograms of F-actin amount in the corresponding sample. Numbers indicate the median fluorescence intensity. Bar graphs on the bottom right represent the mean ± SD of the median fluorescence intensity values obtained in three different experiments. * p < 0.05 and ** p < 0.01 vs. Mock transfected cells. ...View More

Matarrese, P., Vona, R., Ascione, B., Paggi, M. G., & Mileo, A. M. (2021). Physical interaction between HPV16E7 and the actin-binding protein gelsolin regulates epithelial-mesenchymal transition via HIPPO-YAP axis. Cancers, 13(2), 353.

Mouse Anti-AMOT Recombinant Antibody (CBYC-A564) in Immunoblot

Immunoblots of cytoplasmic AMOT, AMOTL1, and AMOTL2 (upper panels) and nuclear YAP and TAZ (lower panels) after 24- or 72-h treatment with G007-LK (1 μM) compared to controls (0.01% DMSO). Actin and lamin B1 document protein loading and representative data from two or more independent experiments are shown. ...View More

Mygland, L., Brinch, S. A., Strand, M. F., Olsen, P. A., Aizenshtadt, A., Lund, K., ... & Waaler, J. (2021). Identification of response signatures for tankyrase inhibitor treatment in tumor cell lines. Iscience, 24(7).

Mouse Anti-AMOT Recombinant Antibody (CBYC-A564) in WB

Protein levels of WNT downstream targets were determined by western blotting. ...View More

Yang, J., Zhang, X., Chen, Z., Shen, Y., Wang, F., Wang, Y., ... & Yang, J. (2019). Angiomotin-p130 inhibits β-catenin stability by competing with Axin for binding to tankyrase in breast cancer. Cell Death & Disease, 10(3), 179.

Mouse Anti-AMOT Recombinant Antibody (CBYC-A564) in IHC

IHC analysis of protein expression of Amot-p130, E-cadherin, and vimentin in xenograft tissues. Scale bar = 200 µm. All P values were calculated by paired Student's t test. ...View More

Mouse Anti-AMOT Recombinant Antibody (CBYC-A564) in IP

O-GlcNAcylation of AMOT in liver cancer cells.
(A) Interactions between O-GlcNAc and Hippo signaling core factors. Endogenous O-GlcNAc was immunoprecipitated by anti-O-GlcNAc antibodies in Bel-7402 and SMMC7721 cells. Co-immunoprecipitated proteins were analyzed using indicated antibodies.(B) Enzymatic labelling of O-GlcNAc in AMOT proteins. Endogenous AMOT was immunoprecipitated by anti-AMOT antibodies in Bel-7402 and SMMC-7721 cells. The biotin or TAMRA labeled O-GlcNAcylated AMOT was measured by anti-Streptavidin or anti-TAMRA. (C) Interactions among AMOT, OGT and O-GlcNAc. Endogenous AMOT was immunoprecipitated by anti-AMOT antibodies in Bel-7402 and SMMC-7721 cells. Co-immunoprecipitated proteins were analyzed using indicated antibodies. ...View More

Liu, Y., Lu, Z., Shi, Y., & Sun, F. (2018). AMOT is required for YAP function in high glucose induced liver malignancy. Biochemical and biophysical research communications, 495(1), 1555-1561.

Mouse Anti-AMOT Recombinant Antibody (CBYC-A564) in IF

Glucose regulated YAP subcellular localizations via AMOT. Bel-7402 and SMMC-7721 cells were cultured in media containing the indicated concentrations of glucose for 24 h with or without knockdown of AMOT. Co-localization of AMOT and YAP was measured by confocal microscopy using anti-AMOT and anti-YAP antibodies. Scale bar, 20 mm. ...View More

Liu, Y., Lu, Z., Shi, Y., & Sun, F. (2018). AMOT is required for YAP function in high glucose induced liver malignancy. Biochemical and biophysical research communications, 495(1), 1555-1561.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A564

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 174-279 mapping within an internal region of Angiomotin of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:30-1:3,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Angiomotin

Introduction

AMOT belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. AMOT is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placent

Entrez Gene ID

154796

UniProt ID

Q4VCS5

Alternative Names

Angiomotin; Angiomotin P130 Isoform; Angiomotin P80 Isoform; KIAA1071;

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

AMOT Matched Antibody Pair (072) (CAT#: APMAB-072LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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