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Mouse Anti-AMOT Recombinant Antibody (G-12) (CBMAB-A2551-YC)

Provided herein is a Mouse monoclonal antibody against Human Angiomotin. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all AMOT antibodies
Published Data
Mouse Anti-AMOT Recombinant Antibody (G-12) in WB
Lower Amot expression in the cerebellum of Amot fl/fl;Syn-Cre P30 mice, analyzed by western blot. ...View More
Rojek, K. O., Krzemień, J., Doleżyczek, H., Boguszewski, P. M., Kaczmarek, L., Konopka, W., ... & Prószyński, T. J. (2019). Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. PLoS biology, 17(5), e3000253.
Mouse Anti-AMOT Recombinant Antibody (G-12) in IF
Sagittal cerebellar sections of Amot fl/fl and Amot fl/fl;Syn-Cre P30 mice that were immunolabeled for calbindin (green) to visualize Purkinje cells and the molecular layer and NeuN (red) to visualize the granule cell layer. Numbers indicate individual lobes. ...View More
Rojek, K. O., Krzemień, J., Doleżyczek, H., Boguszewski, P. M., Kaczmarek, L., Konopka, W., ... & Prószyński, T. J. (2019). Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. PLoS biology, 17(5), e3000253.

Summary

Host Animal
Mouse
Specificity
Human
Clone
G-12
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 825-847 within an internal region of Angiomotin of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:30-1:3,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Angiomotin
Introduction
AMOT belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. AMOT is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placent
Entrez Gene ID
154796
UniProt ID
Q4VCS5
Alternative Names
Angiomotin; Angiomotin P130 Isoform; Angiomotin P80 Isoform; KIAA1071;
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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