Mouse Anti-BIRC3 Recombinant Antibody (315304) (CBMAB-1214-CN)

Name: Mouse Anti-BIRC3 Recombinant Antibody (315304)
SKU: CBMAB-1214-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-BIRC3 Recombinant Antibody (315304) (CBMAB-1214-CN) in WB

cIAP2 expression level. cIAP2 protein had a lower expression level, in the lung tissues of the patients dying from human infection with H7N9 virus than the control group. ((a) and (b) P<0.05). Te experimental group exhibited a lower cIAP2 mRNA level than the control group. ((c) P<0.05). ...View More

Qin, C., Sai, X. Y., Qian, X. F., Wu, Y., Zou, L. F., Wang, H. M., ... & Yan, Z. (2019). Close relationship between cIAP2 and human ARDS induced by severe H7N9 infection. BioMed research international, 2019.

Mouse Anti-BIRC3 Recombinant Antibody (315304) (CBMAB-1214-CN) in WB

Necroptosis was observed in SM-164 treated alveolar epithelium cells during LPS-induced ARDS. Te mouse alveolar epithelium cell line MLE12 was exposed to SM-164 for 24h with or without LPS for 24h. RIPK3 and MLKL were downregulated along with the inhibition of cIAP2 by SM-164 during LPS-induced ARDS. ...View More

Mouse Anti-BIRC3 Recombinant Antibody (315304) (CBMAB-1214-CN) in IHC

BIRC3 is expressed at higher level in the tumor cell niche compared to the vascular niche in GBM. Human GBM tissue microarray was stained for BIRC3 (Brown). BIRC3 expression can be compared between GBM tumor cell niche and vascular endothelial cell niche (A,B). Please note the focus of microvascular proliferation which shows negligible BIRC3 expression (Red arrow). ...View More

Wang, D., Berglund, A. E., Kenchappa, R. S., MacAulay, R. J., Mulé, J. J., & Etame, A. B. (2017). BIRC3 is a biomarker of mesenchymal habitat of glioblastoma, and a mediator of survival adaptation in hypoxia-driven glioblastoma habitats. Scientific reports, 7(1), 9350.

Mouse Anti-BIRC3 Recombinant Antibody (315304) (CBMAB-1214-CN) in IHC

GBM xenografs were established by by injecting 2×106 U87 cells on the fank of 6-8 week nude mice. At 6 weeks, mice were sacrifced and xenografs were assessed for BIRC3 (brown) and CA9 (pink) or HIF-1α (pink) expression by IHC. ...View More

Mouse Anti-BIRC3 Recombinant Antibody (315304) (CBMAB-1214-CN) in WB

BIRC3 protein level were also assessed following knockdown of HIF-1α+/− hypoxia (n=3 independent experiments). ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

315304

Application

WB, IHC

Immunogen

E. coli-derived recombinant human cIAP‑2/HIAP‑1 Asn2-Ser604.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	0.5 μg/ml
IHC	8-25 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Concentration

LYOPH

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Baculoviral IAP Repeat Containing 3

Introduction

This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein is a multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified.

Entrez Gene ID

Human	330
Mouse	11796

UniProt ID

Human	Q13489
Mouse	O08863

Alternative Names

AIP1; API2; MIHC; CIAP2; HAIP1; HIAP1; MALT2; RNF49; c-IAP2

Function

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8.

Biological Process

Cell surface receptor signaling pathway Source: ProtInc
I-kappaB kinase/NF-kappaB signaling Source: Reactome
MyD88-independent toll-like receptor signaling pathway Source: Reactome
Negative regulation of apoptotic process Source: GO_Central
Negative regulation of necroptotic process Source: GO_Central
NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of protein ubiquitination Source: UniProtKB
Protein deubiquitination Source: Reactome
Regulation of apoptotic process Source: UniProtKB
Regulation of cell cycle Source: GO_Central
Regulation of cysteine-type endopeptidase activity Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Regulation of innate immune response Source: UniProtKB
Regulation of necroptotic process Source: UniProtKB
Regulation of nucleotide-binding oligomerization domain containing signaling pathway Source: UniProtKB
Regulation of RIG-I signaling pathway Source: UniProtKB
Regulation of toll-like receptor signaling pathway Source: UniProtKB
Regulation of tumor necrosis factor-mediated signaling pathway Source: Reactome
TRIF-dependent toll-like receptor signaling pathway Source: Reactome
Tumor necrosis factor-mediated signaling pathway Source: Reactome

Cellular Location

Cytoplasm; Nucleus

Involvement in disease

A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.

PTM

Auto-ubiquitinated and degraded by the proteasome in apoptotic cells.

Cai, M., Shao, J., Yung, B., Wang, Y., Gao, N. N., Xu, X., ... & Yao, D. B. (2022). Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury. Neural Regeneration Research, 17(4), 845.

Makuch-Kocka, A., Kocki, J., Brzozowska, A., Bogucki, J., Kołodziej, P., Płachno, B. J., & Bogucka-Kocka, A. (2021). The BIRC Family Genes Expression in Patients with Triple Negative Breast Cancer. International journal of molecular sciences, 22(4), 1820.

Li, S., Yang, P., Xu, L., & Li, M. (2021). Blocking of Birc3/TLR4/Myd88 signaling protects carbapenem-resistant klebsiella pneumoniae in a mouse model of infection. Transplant Immunology, 101464.

Frazzi, R. (2021). BIRC3 and BIRC5: multi‐faceted inhibitors in cancer. Cell & Bioscience, 11(1), 1-14.

Rath-Deschner, B., Nogueira, A. V. B., Memmert, S., Nokhbehsaim, M., Augusto Cirelli, J., Eick, S., ... & Damanaki, A. (2021). Regulation of anti-apoptotic SOD2 and BIRC3 in periodontal cells and tissues. International Journal of Molecular Sciences, 22(2), 591.

Chen, H. D., Guo, N., Song, S. S., Chen, C. H., Miao, Z. H., & He, J. X. (2020). Novel mutations in BRCA2 intron 11 and overexpression of COX-2 and BIRC3 mediate cellular resistance to PARP inhibitors. American journal of cancer research, 10(9), 2813.

Carusone, T. M., Cardiero, G., Cerreta, M., Mandrich, L., Moran, O., Porzio, E., ... & Manco, G. (2020). WTAP and BIRC3 are involved in the posttranscriptional mechanisms that impact on the expression and activity of the human lactonase PON2. Cell death & disease, 11(5), 1-17.

Fu, P. Y., Hu, B., Ma, X. L., Yang, Z. F., Yu, M. C., Sun, H. X., ... & Zhou, J. (2019). New insight into BIRC3: a novel prognostic indicator and a potential therapeutic target for liver cancer. Journal of cellular biochemistry, 120(4), 6035-6045.

Cao, J., Qiu, J., Wang, X., Lu, Z., Wang, D., Feng, H., ... & Wang, L. (2018). Identification of microRNA-124 in regulation of Hepatocellular carcinoma through BIRC3 and the NF-κB pathway. Journal of Cancer, 9(17), 3006.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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