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Mouse Anti-ARIH1 Recombinant Antibody (C-7) (CBMAB-A3563-YC)

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Published Data
Mouse Anti-ARIH1 Recombinant Antibody (C-7) CBMAB-A3563-YC in Immunoblot
Mouse Anti-ARIH1 Recombinant Antibody (C-7) CBMAB-A3563-YC in Immunoblot
Immunoblots (IB) analysis for the ARIH1 levels in MDA-MB-231 and 4T1 cells treated with 1 µM ACY738 at the indicated time points. The numbers under the blots represent the gray scale quantification (ARIH1/Actin and ARIH1/Tubulin). ...View More
Liu, X., Cen, X., Wu, R., Chen, Z., Xie, Y., Wang, F., ... & Xia, H. (2023). ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade. Nature Communications, 14(1), 4066.
Mouse Anti-ARIH1 Recombinant Antibody (C-7) CBMAB-A3563-YC in IF
Mouse Anti-ARIH1 Recombinant Antibody (C-7) CBMAB-A3563-YC in IF
Immunofluorescent staining of DNA-PKcs and Lamp2 in CTRL and Arih1-WT-OE 4T1 cells and their quantifications. The nuclei were stained with DAPI. Scale bar, 10 μm; insets: Scale bar, 2 μm. Data represent means ± SEM, ****P < 0.0001. Each dot in the graph represents the percentage of counted cells with co-localization in each sample, and the total number of counted cells in each group is as follows: CTRL group (n = 381 cells), Arih1-WT-OE group (n = 321 cells). ...View More
Liu, X., Cen, X., Wu, R., Chen, Z., Xie, Y., Wang, F., ... & Xia, H. (2023). ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade. Nature Communications, 14(1), 4066.
Mouse Anti-ARIH1 Recombinant Antibody (C-7) CBMAB-A3563-YC in IP
Mouse Anti-ARIH1 Recombinant Antibody (C-7) CBMAB-A3563-YC in IP
Co-IP analysis for the interaction of ARIH1 and endogenous PD-L1 in HEK293T cells transfected with HA-ARIH1; HA-tagged empty vector (HA-EV) was transfected as a negative control. ...View More
Wu, Y., Zhang, C., Liu, X., He, Z., Shan, B., Zeng, Q., ... & Xia, H. (2021). ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.
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Basic Information

Host Animal
Mouse
Clone
C-7
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 374-557 mapping at the C-terminus of ARIH1 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Ariadne RBR E3 Ubiquitin Protein Ligase 1
Introduction
ARIH1 is an E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. ARIH1 acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (C
Entrez Gene ID
Human25820
Mouse23806
Rat300756
UniProt ID
HumanQ9Y4X5
MouseQ9Z1K5
RatD3ZXL1
Alternative Names
Ariadne RBR E3 Ubiquitin Protein Ligase 1; Ubiquitin-Conjugating Enzyme E2-Binding Protein 1; Ariadne, Drosophila, Homolog Of; Protein Ariadne-1 Homolog; UbcM4-Interacting Protein; UbcH7-Binding Protein; Monocyte Protein 6; UBCH7BP; H7-AP2; ARI-1; HHARI;
Function
E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686).
Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed:27565346).
The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed:27565346).
E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed:24076655, PubMed:27565346).
Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed:25624349).
According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349).
According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed:14623119).
Acts as the ligase involved in ISGylation of EIF4E2 (PubMed:17289916).
In vitro, controls the degradation of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex member SUN2 and may therefore have a role in the formation and localization of the LINC complex, and as a consequence, nuclear subcellular localization and nuclear morphology (PubMed:29689197).
Biological Process
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Protein polyubiquitination Source: GO_Central
Protein ubiquitination Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: GO_Central
Cellular Location
Nucleus; Cajal body; Cytoplasm. Mainly cytoplasmic (PubMed:11278816). Present in Lewy body (PubMed:21590270).
Involvement in disease
Defects in ARIH1 have been found in several individuals with thoracic aortic aneurysms and cerebrovascular disease.

Horn-Ghetko, D., Krist, D. T., Prabu, J. R., Baek, K., Mulder, M. P., Klügel, M., ... & Schulman, B. A. (2021). Ubiquitin ligation to F-box protein targets by SCF–RBR E3–E3 super-assembly. Nature, 590(7847), 671-676.

Kelsall, I. R., Kristariyanto, Y. A., Knebel, A., Wood, N. T., Kulathu, Y., & Alpi, A. F. (2019). Coupled monoubiquitylation of the co-E3 ligase DCNL1 by Ariadne-RBR E3 ubiquitin ligases promotes cullin-RING ligase complex remodeling. Journal of Biological Chemistry, 294(8), 2651-5314.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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