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Mouse Anti-ARIH1 Recombinant Antibody (C-7) (CBMAB-A3563-YC)

Provided herein is a Mouse monoclonal antibody against Human Ariadne RBR E3 Ubiquitin Protein Ligase 1. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ARIH1 antibodies
Published Data
Mouse Anti-ARIH1 Recombinant Antibody (C-7) CBMAB-A3563-YC in Immunoblot
Immunoblots (IB) analysis for the ARIH1 levels in MDA-MB-231 and 4T1 cells treated with 1 µM ACY738 at the indicated time points. The numbers under the blots represent the gray scale quantification (ARIH1/Actin and ARIH1/Tubulin). ...View More
Liu, X., Cen, X., Wu, R., Chen, Z., Xie, Y., Wang, F., ... & Xia, H. (2023). ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade. Nature Communications, 14(1), 4066.
Mouse Anti-ARIH1 Recombinant Antibody (C-7) CBMAB-A3563-YC in IF
Immunofluorescent staining of DNA-PKcs and Lamp2 in CTRL and Arih1-WT-OE 4T1 cells and their quantifications. The nuclei were stained with DAPI. Scale bar, 10 μm; insets: Scale bar, 2 μm. Data represent means ± SEM, ****P < 0.0001. Each dot in the graph represents the percentage of counted cells with co-localization in each sample, and the total number of counted cells in each group is as follows: CTRL group (n = 381 cells), Arih1-WT-OE group (n = 321 cells). ...View More
Liu, X., Cen, X., Wu, R., Chen, Z., Xie, Y., Wang, F., ... & Xia, H. (2023). ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade. Nature Communications, 14(1), 4066.
Mouse Anti-ARIH1 Recombinant Antibody (C-7) CBMAB-A3563-YC in IP
Co-IP analysis for the interaction of ARIH1 and endogenous PD-L1 in HEK293T cells transfected with HA-ARIH1; HA-tagged empty vector (HA-EV) was transfected as a negative control. ...View More
Wu, Y., Zhang, C., Liu, X., He, Z., Shan, B., Zeng, Q., ... & Xia, H. (2021). ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
C-7
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 374-557 mapping at the C-terminus of ARIH1 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Ariadne RBR E3 Ubiquitin Protein Ligase 1
Introduction
ARIH1 is an E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. ARIH1 acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (C
Entrez Gene ID
Human25820
Mouse23806
Rat300756
UniProt ID
HumanQ9Y4X5
MouseQ9Z1K5
RatD3ZXL1
Alternative Names
Ariadne RBR E3 Ubiquitin Protein Ligase 1; Ubiquitin-Conjugating Enzyme E2-Binding Protein 1; Ariadne, Drosophila, Homolog Of; Protein Ariadne-1 Homolog; UbcM4-Interacting Protein; UbcH7-Binding Protein; Monocyte Protein 6; UBCH7BP; H7-AP2; ARI-1; HHARI;
Function
E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686).
Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed:27565346).
The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed:27565346).
E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed:24076655, PubMed:27565346).
Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed:25624349).
According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349).
According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed:14623119).
Acts as the ligase involved in ISGylation of EIF4E2 (PubMed:17289916).
In vitro, controls the degradation of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex member SUN2 and may therefore have a role in the formation and localization of the LINC complex, and as a consequence, nuclear subcellular localization and nuclear morphology (PubMed:29689197).
Biological Process
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Protein polyubiquitination Source: GO_Central
Protein ubiquitination Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: GO_Central
Cellular Location
Nucleus; Cajal body; Cytoplasm. Mainly cytoplasmic (PubMed:11278816). Present in Lewy body (PubMed:21590270).
Involvement in disease
Defects in ARIH1 have been found in several individuals with thoracic aortic aneurysms and cerebrovascular disease.
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For research use only. Not intended for any clinical use.

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