Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) (CBMAB-P0070-YC)

Provided herein is a Mouse monoclonal antibody against Human Rho GTPase Activating Protein 5. The antibody can be used for immunoassay techniques, such as WB, IF.
See all ARHGAP5 antibodies

Published Data

Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) CBMAB-P0070-YC in WB

The ARHGAP5 expression in HTR8/SVneo cells was analyzed by Western blot analysis at the mRNA and protein levels, respectively. ...View More

Taga, S., Hayashi, M., Nunode, M., Nakamura, N., & Ohmichi, M. (2022). miR-486-5p inhibits invasion and migration of HTR8/SVneo trophoblast cells by down-regulating ARHGAP5. Placenta, 123, 5-11.

Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) CBMAB-P0070-YC in IP

Co-IP assay detected binding of both RhoE and RhoEα to Syx and p190RhoGAP. HEK293 cells were transfected with indicated plasmids. Twenty four hours after transfection, cell lysates were used for Co-IP assay with anti-flag antibody. ...View More

Dai, Y., Luo, W., Yue, X., Ma, W., Wang, J., & Chang, J. (2020). Identification and characterization of a new isoform of small GTPase RhoE. Communications Biology, 3(1), 572.

Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) CBMAB-P0070-YC in IF

Immunofluorescence images of fixed RAW 264.7 macrophages challenged with C3biopsonized beads for 45 minutes and stained with phalloidin for F-actin and antibodies to RhoA, p190RhoGAP, p34 Arp2/3 or WASP. Arrows indicate F-actin flashing phagosomes, while arrowheads indicate non-flashing phagosomes. ...View More

Poirier, M. B., Fiorino, C., Rajasekar, T. K., & Harrison, R. E. (2020). F-actin flashes on phagosomes mechanically deform contents for efficient digestion in macrophages. Journal of cell science, 133(12), jcs239384.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

54/P190-B

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 1091-1250 mapping within an internal region of Rho GAP p190-B of human origin.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Rho GTPase Activating Protein 5

Introduction

Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP.

Entrez Gene ID

Human	23092
Dog	490642
Rat	307459

UniProt ID

Human	Q9UNA1
Dog	A0A5F4CSE9
Rat	Q9ERC1

Alternative Names

GFI2; RhoGAP5; p190-B; p190BRhoGAP

Function

GTPase-activating protein for Rho family members (PubMed:8537347).

Biological Process

Cell adhesion Source: ProtInc
Mammary gland development Source: Ensembl
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: ProtInc

Cellular Location

Cell membrane; Cytoplasm. Also membrane-associated in a fibrillar pattern that colocalizes with the alpha5-beta1 integrin receptor (ITGA5/ITGB1) for fibronectin.

References

Wang, C. L., Li, J. C., Zhou, C. X., Ma, C. N., Wang, D. F., Wo, L. L., ... & Zhao, Q. (2021). Long non-coding RNA ARHGAP5-AS1 inhibits migration of breast cancer cell via stabilizing SMAD7 protein. Breast Cancer Research and Treatment, 1-13.

Tian, T., Chen, Z. H., Zheng, Z., Liu, Y., Zhao, Q., Liu, Y., ... & Deng, W. (2020). Investigation of the role and mechanism of ARHGAP5-mediated colorectal cancer metastasis. Theranostics, 10(13), 5998.

Héraud, C., Pinault, M., Lagrée, V., & Moreau, V. (2019). p190RhoGAPs, the ARHGAP35-and ARHGAP5-encoded proteins, in health and disease. Cells, 8(4), 351.

Li, C., Jia, L., Yu, Y., & Jin, L. (2019). Lactic acid induced microRNA-744 enhances motility of SiHa cervical cancer cells through targeting ARHGAP5. Chemico-biological interactions, 298, 86-95.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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