Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-8-oxoguanine Recombinant Antibody (V2-7719) (CBMAB-1898CQ)


See all 8-oxoguanine antibodies
Online Inquiry
Published Data
Mouse Anti-8-oxoguanine Recombinant Antibody (483.15) in IF
The mitochondrial ROS and oxidative DNA damage were assessed by 8-OxoG staining. ...View More
Li, H., Zhu, J., Xu, Y. W., Mou, F. F., Shan, X. L., Wang, Q. L., ... & Guo, H. D. (2022). Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction. Redox Biology, 54, 102384.
Mouse Anti-8-oxoguanine Recombinant Antibody (483.15) in IF
Higher nuclear and perinuclear 8-oxoguanine (8-oxoG) positivity (green color) for Rpl5- and Rps19-deficient cells compared to control cells. Cell nuclei were counterstained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI, blue color). ...View More
Kapralova, K., Jahoda, O., Koralkova, P., Gursky, J., Lanikova, L., Pospisilova, D., ... & Horvathova, M. (2020). Oxidative DNA damage, inflammatory signature, and altered erythrocytes properties in diamond-blackfan anemia. International Journal of Molecular Sciences, 21(24), 9652.
Mouse Anti-8-oxoguanine Recombinant Antibody (483.15) in IF
S. aureus induce 8-oxoG DNA lesion in the host cells. Either HeLa or MG-63 cells were exposed to S. aureus MW2 strain at MOI 1:50 for 2 h followed by antibiotic treatment as described. As the positive control 50 µM etoposide was used. After 6 h and 20 h of post-infection cells were immunostained with mouse anti 8-oxoG DNA lesion antibody, followed by incubation with m-IgGκ BP-FITC at dilution 1:50 for 2 h at room temperature (green staining, the red arrow). Nuclear DNA were labeled with DAPI (blue staining). Samples were viewed with Leica SP8 laser-scanning microscope equipped with immersion objective 63× plan Apo-NA 1.4 driven by the LAS software. Scale bar: 5 µm. ...View More
Deplanche, M., Mouhali, N., Nguyen, M. T., Cauty, C., Ezan, F., Diot, A., ... & Berkova, N. (2019). Staphylococcus aureus induces DNA damage in host cell. Scientific Reports, 9(1), 7694.
Mouse Anti-8-oxoguanine Recombinant Antibody (483.15) in IF
BoHV1 infection increased the levels of oxidative DNA damage marker 8-oxoG. (A) MDBK cells were infected by either BoHV-1 or UV-inactivated virus for designated periods. The production of 8-oxoG was detected with immunofluorescence assay. Images shown were represent of three independent experiments (Magnification ×200). (B) Fluorescence intensity of three images from each sample was analyzed with software image J. The relative fold change was calculated by normalization to the value of 12 h after infection which was arbitrarily set as 1. NA, not analyzed. (C) Total RNA was prepared at 24 and 36 h after infection in MDBK cells, and the mRNA levels of OGG1 were measured by qRT-PC. Each analysis was compared with that of uninfected control which was arbitrarily set as 100%. Data represent three independent experiments. Significance was assessed with the student t test (*, P < 0.05). (D) MDBK cells in 60 mm dishes were mock infected or infected with BoHV-1 (MOI = 0.1) for 12, 24 and 36 h. The cell lysates were prepared for Western blotting to detect the expression of viral protein VP16. Data shown are representative of three independent experiments. ...View More
Zhu, L., Fu, X., Yuan, C., Jiang, X., & Zhang, G. (2018). Induction of oxidative DNA damage in bovine herpesvirus 1 infected bovine kidney cells (MDBK cells) and human tumor cells (A549 cells and U2OS cells). Viruses, 10(8), 393.

Summary

Host Animal
Mouse
Specificity
Chemical
Clone
V2-7719
Antibody Isotype
IgM
Application
ELISA, ICC, IHC

Basic Information

Immunogen
8-oxoguanine adsorbed on to alumina
Host Species
Mouse
Specificity
Chemical
Antibody Isotype
IgM
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
8-oxoguanine
Introduction
Oxoguanine 8 (8-Oxoguanine) is a mutagenic oxidative damage product of guanine. Guanine is the main target for reactive oxygen species in DNA, with 8-oxoguanine being the most frequent base lesion. Thus formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1.
Alternative Names
8-Oxoguanine; 8-hydroxyguanine; 8-oxo-Gua; OH8Gua
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-8-oxoguanine Recombinant Antibody (V2-7719)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry