Mouse Anti-CSTF2 Recombinant Antibody (3D1-3A6) (CBMAB-C5862-LY)

This product is antibody recognizes CSTF2. The antibody 3D1-3A6 immunoassay techniques such as: ELISA, IHC-P, WB.
See all CSTF2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3D1-3A6

Antibody Isotype

IgG1, κ

Application

ELISA, IHC-P, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cleavage Stimulation Factor Subunit 2

Introduction

CSTF1 (Cleavage Stimulation Factor Subunit 1) is a Protein Coding gene. Among its related pathways are mRNA Splicing - Major Pathway and RNA Polymerase II Transcription Termination. Gene Ontology (GO) annotations related to this gene include RNA binding.

Entrez Gene ID

1478

UniProt ID

P33240

Alternative Names

Cleavage Stimulation Factor Subunit 2; Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 2, 64kD; Cleavage Stimulation Factor 64 KDa Subunit; CF-1 64 KDa Subunit; CSTF 64 KDa Subunit; CstF-64; Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 2, 64kDa; Cleava

Function

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).

Biological Process

mRNA 3'-end processing Source: Reactome
mRNA polyadenylation Source: InterPro
mRNA splicing, via spliceosome Source: Reactome
Pre-mRNA cleavage required for polyadenylation Source: GO_Central
Termination of RNA polymerase II transcription Source: Reactome
tRNA splicing, via endonucleolytic cleavage and ligation Source: Reactome

Cellular Location

Nucleus. Localized with DDX1 in cleavage bodies.

References

Masoumzadeh, E., Grozdanov, P. N., Jetly, A., MacDonald, C. C., & Latham, M. P. (2022). Electrostatic interactions between CSTF2 and pre-mRNA drive cleavage and polyadenylation. Biophysical Journal.

Mamoor, S. (2021). Differential expression of cleavage stimulation factor subunit 2 in cancers of the breast.

Grozdanov, P. N., Masoumzadeh, E., Kalscheuer, V. M., Bienvenu, T., Billuart, P., Delrue, M. A., ... & MacDonald, C. C. (2020). A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3′ end processing is associated with intellectual disability in humans. Nucleic acids research, 48(17), 9804-9821.

Chen, X., Zhang, J. X., Luo, J. H., Wu, S., Yuan, G. J., Ma, N. F., ... & Xie, D. (2018). CSTF2-induced shortening of the RAC1 3′ UTR promotes the pathogenesis of urothelial carcinoma of the bladder. Cancer research, 78(20), 5848-5862.

Popow, J., Wieshofer, C., Schlattl, A., Woehrle, S., Neumüller, R., Petronczki, M. P., ... & Pearson, M. (2018). Synthetic lethality between CSTF2 and CSTF2T in lung adenocarcinoma and melanoma cell lines.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

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Mouse Anti-CSTF2 Recombinant Antibody (CBXC-1796)

Rabbit Anti-CSTF2 Recombinant Antibody (EG854)

Mouse Anti-CSTF2 Recombinant Antibody (CBYY-C2223)

Mouse Anti-CSTF2 Antibody (1A5)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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