Sign in or Register   Sign in or Register
  |  

Mouse Anti-CYB5A Recombinant Antibody (CBYY-C3177) (CBMAB-C4620-YY)

This product is mouse antibody that recognizes CYB5A. The antibody CBYY-C3177 can be used for immunoassay techniques such as: ELISA, WB, IHC
See all CYB5A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-C3177
Antibody Isotype
IgG1, κ
Application
ELISA, WB, IHC

Basic Information

Immunogen
Human Recombinant Hsp70 overexpressed in E. coli
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Protein A purified
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytochrome B5 Type A
Entrez Gene ID
1528
UniProt ID
P00167
Alternative Names
Coiled-Coil Domain Containing 93;
Function
Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.
Biological Process
L-ascorbic acid metabolic process Source: Reactome
Protein insertion into ER membrane Source: Reactome
Response to cadmium ion Source: Ensembl
Cellular Location
Isoform 1: Endoplasmic reticulum membrane; Microsome membrane
Isoform 2: Cytoplasm
Involvement in disease
Methemoglobinemia and ambiguous genitalia (METAG):
An autosomal recessive disorder characterized by sex steroid deficiency but normal glucocorticoid and mineralocorticoid reserve, male undermasculinization, absent or disturbed pubertal development, decreased levels of erythrocyte cytochrome B5, and excessive amounts of methemoglobin in blood cells resulting in cyanosis and hypoxia.
Topology
Helical: 109-131

Day, S. E., Traurig, M., Kumar, P., Piaggi, P., Koroglu, C., Kobes, S., ... & Baier, L. J. (2022). Functional variants in cytochrome b5 type A (CYB5A) are enriched in Southwest American Indian individuals and associate with obesity. Obesity.

Kaluzhskiy, L. A., Ershov, P. V., Kurpedinov, K. S., Sonina, D. S., Yablokov, E. O., Shkel, T. V., ... & Ivanov, A. S. (2020). SPR Analysis of Protein-Protein Interactions Involving Cytochromes P450 and Cytochrome b5 Integrated into Lipid Membrane. Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry, 14(2), 168-173.

Leung, M. T., Cheung, H. N., Iu, Y. P., Choi, C. H., Tiu, S. C., & Shek, C. C. (2020). Isolated 17, 20-lyase deficiency in a CYB5A mutated female with normal sexual development and fertility. Journal of the Endocrine Society, 4(2), bvz016.

Day, S. E., Traurig, M., Piaggi, P., Kumar, P., Kobes, S., Hanson, R. L., ... & Baier, L. (2020). 2018-P: Whole-Genome Sequence Identifies Potentially Functional Variants in Cytochrome B5 Type A (CYB5A) that Associate with Obesity in Southwest American Indians. Diabetes, 69(Supplement_1).

Squires, E. J., Gray, M. A., & Lou, Y. (2019). Effect of mutations in porcine CYB5A and CYP17A1 on the metabolism of pregnenolone. The Journal of Steroid Biochemistry and Molecular Biology, 195, 105469.

Yoo, S. E., Yi, M., Kim, W. Y., Cho, S. A., Lee, S. S., Lee, S. J., & Shin, J. G. (2019). Influences of cytochrome b5 expression and its genetic variant on the activity of CYP2C9, CYP2C19 and CYP3A4. Drug Metabolism and Pharmacokinetics, 34(3), 201-208.

Jaffey, J. A., Harmon, M. R., Villani, N. A., Creighton, E. K., Johnson, G. S., Giger, U., & Dodam, J. R. (2017). Long‐term treatment with methylene blue in a dog with hereditary methemoglobinemia caused by cytochrome b5 reductase deficiency. Journal of veterinary internal medicine, 31(6), 1860-1865.

Bhatt, M. R., Khatri, Y., Rodgers, R. J., & Martin, L. L. (2017). Role of cytochrome b5 in the modulation of the enzymatic activities of cytochrome P450 17α-hydroxylase/17, 20-lyase (P450 17A1). The Journal of Steroid Biochemistry and Molecular Biology, 170, 2-18.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CYB5A Recombinant Antibody (CBYY-C3177)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare