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Rabbit Anti-CYP2E1 Recombinant Antibody (EG898) (CBMAB-EN1069-LY)

The product is antibody recognizes CYP2E1. The antibody EG898 immunoassay techniques such as: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000.
See all CYP2E1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG898
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from C-terminal of human Cytochrome P450 2E1.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 2 Subfamily E Member 1
Introduction
CYP2E1 (Cytochrome P450 Family 2 Subfamily E Member 1) is a Protein Coding gene. Diseases associated with CYP2E1 include Alcohol Abuse and Fatty Liver Disease. Among its related pathways are Caffeine Pathway, Pharmacokinetics and Acetaminophen Pathway (therapeutic doses), Pharmacokinetics. Gene Ontology (GO) annotations related to this gene include enzyme binding and iron ion binding. An important paralog of this gene is CYP2C9.
Entrez Gene ID
Human1571
Mouse13106
Rat25086
UniProt ID
HumanP05181
MouseQ05421
RatP05182
Alternative Names
Cytochrome P450 Family 2 Subfamily E Member 1; Cytochrome P450, Subfamily IIE (Ethanol-Inducible), Polypeptide 1; Cytochrome P450, Family 2, Subfamily E, Polypeptide 1; 4-Nitrophenol 2-Hydroxylase; Cytochrome P450-J; CYPIIE1; CYP2E; Flavoprotein-Linked Monooxygenase; Microsomal Monooxygenase;
Function
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed:10553002, PubMed:18577768).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10553002, PubMed:18577768).

Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed:10553002, PubMed:18577768).

May be involved in the oxidative metabolism of xenobiotics (Probable).
Biological Process
4-nitrophenol metabolic process Source: UniProtKB
Benzene metabolic process Source: Reactome
Carbon tetrachloride metabolic process Source: Reactome
Drug metabolic process Source: BHF-UCL
Epoxygenase P450 pathway Source: GO_Central
Exogenous drug catabolic process Source: GO_Central
Halogenated hydrocarbon metabolic process Source: Reactome
Heterocycle metabolic process Source: BHF-UCL
Lipid hydroxylation Source: UniProtKB
Long-chain fatty acid biosynthetic process Source: Reactome
Monoterpenoid metabolic process Source: BHF-UCL
Organic acid metabolic process Source: GO_Central
Response to bacterium Source: Ensembl
Response to ethanol Source: Ensembl
Response to organonitrogen compound Source: Ensembl
Response to ozone Source: Ensembl
Steroid metabolic process Source: BHF-UCL
Triglyceride metabolic process Source: Ensembl
Xenobiotic metabolic process Source: GO_Central
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane; Mitochondrion inner membrane. Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein.

Wang, K., Tan, W., Liu, X., Deng, L., Huang, L., Wang, X., & Gao, X. (2021). New insight and potential therapy for NAFLD: CYP2E1 and flavonoids. Biomedicine & Pharmacotherapy, 137, 111326.

Diesinger, T., Buko, V., Lautwein, A., Dvorsky, R., Belonovskaya, E., Lukivskaya, O., ... & Haehner, T. (2020). Drug targeting CYP2E1 for the treatment of early-stage alcoholic steatohepatitis. PLoS One, 15(7), e0235990.

Rahman, M. A., Kodidela, S., Sinha, N., Haque, S., Shukla, P. K., Rao, R., & Kumar, S. (2019). Plasma exosomes exacerbate alcohol-and acetaminophen-induced toxicity via CYP2E1 pathway. Scientific reports, 9(1), 1-10.

Davydova, N. Y., Dangi, B., Maldonado, M. A., Vavilov, N. E., Zgoda, V. G., & Davydov, D. R. (2019). Toward a systems approach to cytochrome P450 ensemble: interactions of CYP2E1 with other P450 species and their impact on CYP1A2. Biochemical Journal, 476(23), 3661-3685.

Lin, Q., Kang, X., Li, X., Wang, T., Liu, F., Jia, J., ... & Xue, Y. (2019). NF-κB-mediated regulation of rat CYP2E1 by two independent signaling pathways. PloS one, 14(12), e0225531.

Zeng, T., Zhang, C. L., Zhao, N., Guan, M. J., Xiao, M., Yang, R., ... & Xie, K. Q. (2018). Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver. Redox biology, 14, 295-304.

Gao, J., Wang, Z., Wang, G. J., Zhang, H. X., Gao, N., Wang, J., ... & Qiao, H. L. (2018). Higher CYP2E1 activity correlates with hepatocarcinogenesis induced by diethylnitrosamine. Journal of Pharmacology and Experimental Therapeutics, 365(2), 398-407.

Wang, Y., Yu, D., Tolleson, W. H., Yu, L. R., Green, B., Zeng, L., ... & Ning, B. (2017). A systematic evaluation of microRNAs in regulating human hepatic CYP2E1. Biochemical pharmacology, 138, 174-184.

A Abdelmegeed, M., Ha, S. K., Choi, Y., Akbar, M., & Song, B. J. (2017). Role of CYP2E1 in mitochondrial dysfunction and hepatic injury by alcohol and non-alcoholic substances. Current molecular pharmacology, 10(3), 207-225.

García-Suástegui, W. A., Ramos-Chávez, L. A., Rubio-Osornio, M., Calvillo-Velasco, M., Atzin-Méndez, J. A., Guevara, J., & Silva-Adaya, D. (2017). The Role of CYP2E1 in the Drug Metabolism or Bioactivation in the Brain. Oxidative medicine and cellular longevity, 2017.

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For research use only. Not intended for any clinical use.

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