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Mouse Anti-CYP2J2 Recombinant Antibody (2A9) (CBMAB-C4641-LY)

This product is antibody recognizes CYP2J2. The antibody 2A9 immunoassay techniques such as: FC, IF, WB.
See all CYP2J2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2A9
Antibody Isotype
IgG1
Application
FC, IF, WB

Basic Information

Immunogen
CYP2J2 antibody was raised in mouse using a full length recombinant protein of human CYP2J2 (NP_000766) produced in HEK293T cells, as the immunogen
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
0.5 mg/mL-1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 2 Subfamily J Member 2
Introduction
CYP2J2 (Cytochrome P450 Family 2 Subfamily J Member 2) is a Protein Coding gene. Diseases associated with CYP2J2 include Clopidogrel Resistance. Among its related pathways are Metabolism and Arachidonic acid metabolism. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.
An important paralog of this gene is CYP2D6.
Entrez Gene ID
1573
UniProt ID
P51589
Alternative Names
Cytochrome P450 Family 2 Subfamily J Member 2; Cytochrome P450, Subfamily IIJ (Arachidonic Acid Epoxygenase) Polypeptide 2; Cytochrome P450, Family 2, Subfamily J, Polypeptide 2; Arachidonic Acid Epoxygenase; EC 1.14.14.1; CYPIIJ2;
Function
A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) in the cardiovascular system (PubMed:8631948, PubMed:19965576).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:8631948, PubMed:19965576).

Catalyzes the epoxidation of double bonds of PUFA (PubMed:8631948, PubMed:19965576).

Converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EpETrE), likely playing a major role in the epoxidation of endogenous cardiac arachidonic acid pools (PubMed:8631948).

In endothelial cells, participates in eicosanoids metabolism by converting hydroperoxide species into hydroxy epoxy metabolites. In combination with 15-lipoxygenase metabolizes arachidonic acid and converts hydroperoxyicosatetraenoates (HpETEs) into hydroxy epoxy eicosatrienoates (HEETs), which are precursors of vasodilatory trihydroxyicosatrienoic acids (THETAs). This hydroperoxide isomerase activity is NADPH- and O2-independent (PubMed:19737933).

Catalyzes the monooxygenation of a various xenobiotics, such as danazol, amiodarone, terfenadine, astemizole, thioridazine, tamoxifen, cyclosporin A and nabumetone (PubMed:19923256).

Catalyzes hydroxylation of the anthelmintics albendazole and fenbendazole (PubMed:23959307).

Catalyzes the sulfoxidation of fenbedazole (PubMed:19923256).
Biological Process
Epoxygenase P450 pathway Source: UniProtKB
Exogenous drug catabolic process Source: GO_Central
Fatty acid metabolic process Source: Reactome
Icosanoid metabolic process Source: UniProtKB
Linoleic acid metabolic process Source: BHF-UCL
Organic acid metabolic process Source: GO_Central
Regulation of heart contraction Source: ProtInc
Xenobiotic metabolic process Source: GO_Central
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane

Dai, Y., Xue, T., Ji, H., Zhang, P., Zhang, D., & Qi, Z. (2021). In situ target enzyme-activated near-infrared fluorescent probe: a case study of CYP2J2 using three-fragmentary molecular assembly engineering. Sensors and Actuators B: Chemical, 328, 129034.

Das, A., Weigle, A. T., Arnold, W. R., Kim, J. S., Carnevale, L. N., & Huff, H. C. (2020). CYP2J2 molecular recognition: a new axis for therapeutic design. Pharmacology & therapeutics, 215, 107601.

Sisignano, M., Steinhilber, D., Parnham, M. J., & Geisslinger, G. (2020). Exploring CYP2J2: lipid mediators, inhibitors and therapeutic implications. Drug Discovery Today, 25(9), 1744-1753.

Evangelista, E. A., Aliwarga, T., Sotoodehnia, N., Jensen, P. N., McKnight, B., Lemaitre, R. N., ... & Gharib, S. A. (2020). CYP2J2 modulates diverse transcriptional programs in adult human cardiomyocytes. Scientific reports, 10(1), 1-12.

Arnold, W. R., Weigle, A. T., & Das, A. (2018). Cross-talk of cannabinoid and endocannabinoid metabolism is mediated via human cardiac CYP2J2. Journal of inorganic biochemistry, 184, 88-99.

Aliwarga, T., Evangelista, E. A., Sotoodehnia, N., Lemaitre, R. N., & Totah, R. A. (2018). Regulation of CYP2J2 and EET levels in cardiac disease and diabetes. International journal of molecular sciences, 19(7), 1916.

Zhou, C., Huang, J., Li, Q., Zhan, C., Xu, X., Zhang, X., ... & Wang, D. W. (2018). CYP2J2-derived EETs attenuated ethanol-induced myocardial dysfunction through inducing autophagy and reducing apoptosis. Free Radical Biology and Medicine, 117, 168-179.

Karkhanis, A., Hong, Y., & Chan, E. C. Y. (2017). Inhibition and inactivation of human CYP2J2: Implications in cardiac pathophysiology and opportunities in cancer therapy. Biochemical Pharmacology, 135, 12-21.

Park, S. H., Phuc, N. M., Lee, J., Wu, Z., Kim, J., Kim, H., ... & Liu, K. H. (2017). Identification of acetylshikonin as the novel CYP2J2 inhibitor with anti-cancer activity in HepG2 cells. Phytomedicine, 24, 134-140.

Arnold, W. R., Baylon, J. L., Tajkhorshid, E., & Das, A. (2017). Arachidonic acid metabolism by human cardiovascular CYP2J2 is modulated by doxorubicin. Biochemistry, 56(51), 6700-6712.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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