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Mouse Anti-CYP3A5 Recombinant Antibody (F18P3B6) (CBMAB-C0659-CQ)

This product is a mouse antibody that recognizes CYP3A5. The antibody F18P3B6 can be used for immunoassay techniques such as: WB, ELISA, IHC.
See all CYP3A5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
F18P3B6
Antibody Isotype
IgG1, κ
Application
WB, ELISA, IHC

Basic Information

Immunogen
Ovalbumin-conjugated synthetic peptide DSRDGTLSGE (C-terminal sequence)
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytochrome P450 Family 3 Subfamily A Member 5
Introduction
CYP3A5 (Cytochrome P450 Family 3 Subfamily A Member 5) is a Protein Coding gene. Diseases associated with CYP3A5 include Hypertension, Essential and Tacrolimus Dose Selection. Among its related pathways are Tamoxifen Pathway, Pharmacokinetics and Vinka Alkaloid Pathway, Pharmacokinetics. Gene Ontology (GO) annotations related to this gene include oxidoreductase activity and heme binding. An important paralog of this gene is CYP3A4.
Entrez Gene ID
1577
UniProt ID
P20815
Alternative Names
Cytochrome P450 Family 3 Subfamily A Member 5; Cytochrome P450, Subfamily IIIA (Niphedipine Oxidase), Polypeptide 5; Cytochrome P450, Family 3, Subfamily A, Polypeptide 5; Cytochrome P450 HLp2; Cytochrome P450-PCN3; EC 1.14.14.1; CYPIIIA5; Flavoprotein-Linked Monooxygenase;
Function
A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins (PubMed:2732228, PubMed:10681376, PubMed:11093772, PubMed:12865317).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed:12865317, PubMed:2732228, PubMed:10681376, PubMed:11093772).

Exhibits high catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed:12865317).

Catalyzes 6beta-hydroxylation of the steroid hormones testosterone, progesterone, and androstenedione (PubMed:2732228).

Catalyzes the oxidative conversion of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA) (PubMed:10681376).

Further metabolizes all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in hepatic atRA clearance (PubMed:11093772).

Also involved in the oxidative metabolism of xenobiotics, including calcium channel blocking drug nifedipine and immunosuppressive drug cyclosporine (PubMed:2732228).
Biological Process
Aflatoxin metabolic process Source: Reactome
Alkaloid catabolic process Source: BHF-UCL
Drug catabolic process Source: BHF-UCL
Estrogen metabolic process Source: UniProtKB
Lipid hydroxylation Source: BHF-UCL
Oxidative demethylation Source: BHF-UCL
Retinoic acid metabolic process Source: UniProtKB
Retinol metabolic process Source: UniProtKB-UniPathway
Steroid metabolic process Source: BHF-UCL
Xenobiotic metabolic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane

Rodriguez‐Antona, C., Savieo, J. L., Lauschke, V. M., Sangkuhl, K., Drögemöller, B. I., Wang, D., ... & Gaedigk, A. (2022). PharmVar GeneFocus: CYP3A5. Clinical Pharmacology & Therapeutics.

Lidberg, K. A., Annalora, A. J., Jozic, M., Elson, D. J., Wang, L., Bammler, T. K., ... & Kelly, E. J. (2021). Antisense oligonucleotide development for the selective modulation of CYP3A5 in renal disease. Scientific reports, 11(1), 1-20.

Saiz-Rodríguez, M., Almenara, S., Navares-Gómez, M., Ochoa, D., Román, M., Zubiaur, P., ... & Abad-Santos, F. (2020). Effect of the most relevant CYP3A4 and CYP3A5 polymorphisms on the pharmacokinetic parameters of 10 CYP3A substrates. Biomedicines, 8(4), 94.

Khan, A. R., Raza, A., Firasat, S., & Abid, A. (2020). CYP3A5 gene polymorphisms and their impact on dosage and trough concentration of tacrolimus among kidney transplant patients: a systematic review and meta-analysis. The pharmacogenomics journal, 20(4), 553-562.

Dorji, P. W., Tshering, G., & Na‐Bangchang, K. (2019). CYP2C9, CYP2C19, CYP2D6 and CYP3A5 polymorphisms in South‐East and East Asian populations: a systematic review. Journal of clinical pharmacy and therapeutics, 44(4), 508-524.

Campagne, O., Mager, D. E., Brazeau, D., Venuto, R. C., & Tornatore, K. M. (2018). Tacrolimus population pharmacokinetics and multiple CYP3A5 genotypes in black and white renal transplant recipients. The Journal of Clinical Pharmacology, 58(9), 1184-1195.

Chen, L., & Prasad, G. R. (2018). CYP3A5 polymorphisms in renal transplant recipients: influence on tacrolimus treatment. Pharmacogenomics and personalized medicine, 11, 23.

Andreu, F., Colom, H., Elens, L., van Gelder, T., van Schaik, R. H., Hesselink, D. A., ... & Lloberas, N. (2017). A new CYP3A5* 3 and CYP3A4* 22 cluster influencing tacrolimus target concentrations: a population approach. Clinical pharmacokinetics, 56(8), 963-975.

Lolodi, O., Wang, Y. M., Wright, W. C., & Chen, T. (2017). Differential regulation of CYP3A4 and CYP3A5 and its implication in drug discovery. Current drug metabolism, 18(12), 1095-1105.

Lee, C. T., Chen, J., Kindberg, A. A., Bendriem, R. M., Spivak, C. E., Williams, M. P., ... & Freed, W. J. (2017). CYP3A5 mediates effects of cocaine on human neocorticogenesis: studies using an in vitro 3D self-organized hPSC model with a single cortex-like unit. Neuropsychopharmacology, 42(3), 774-784.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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