Sign in or Register   Sign in or Register
  |  

Recombinant Mouse Anti-CYP46A1 Recombinant Antibody (P6E4) (CBMAB-XB0327-YC)

Provided herein is a Mouse Recombinant Antibody against Cytochrome P450 Family 46 Subfamily A Member 1. The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all CYP46A1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
P6E4
Antibody Isotype
IgG1
Application
ELISA, IHC, WB

Basic Information

Immunogen
Ovalbumin-conjugated Synthetic peptide-PVLCTLRPR (C-terminal sequence)
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
cytochrome P450, family 46, subfamily A, polypeptide 1
Introduction
CYP46A1 (Cytochrome P450 Family 46 Subfamily A Member 1) is a protein coding gene. Diseases associated with CYP46A1 include Anus Disease and Alpha-Methylacyl-Coa Racemase Deficiency. Among its related pathways are Metabolism and Cytochrome P450 - arranged by substrate type. Gene Ontology annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is CYP3A4.
Entrez Gene ID
10858
UniProt ID
Q9Y6A2
Alternative Names
Cytochrome P450 Family 46 Subfamily A Member 1; Cytochrome P450, Subfamily 46 (Cholesterol 24-Hydroxylase); Cytochrome P450, Family 46, Subfamily A, Polypeptide 1; Cytochrome P450 46A1; CYP46; CH24H; Cholesterol 24-Hydroxylase; EC 1.14.14.25; CP46;
Function
P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation (PubMed:10377398, PubMed:14640697, PubMed:25017465, PubMed:18621681).

By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning (By similarity).

Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis (PubMed:12077124, PubMed:14640697, PubMed:28190002).

Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain (PubMed:25017465).

May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole (PubMed:20667828).
Biological Process
Bile acid biosynthetic process Source: Reactome
Cholesterol catabolic process Source: UniProtKB
Nervous system development Source: ProtInc
Progesterone metabolic process Source: UniProtKB
Protein localization to membrane raft Source: ARUK-UCL
Regulation of long-term synaptic potentiation Source: UniProtKB
Sterol metabolic process Source: Reactome
Xenobiotic metabolic process Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane; Postsynapse; Presynapse; Dendrite
Topology
Helical: 3-23

El‐Darzi, N., Mast, N., Petrov, A. M., & Pikuleva, I. A. (2021). 2‐Hydroxypropyl‐β‐cyclodextrin reduces retinal cholesterol in wild‐type and Cyp27a1−/− Cyp46a1−/− mice with deficiency in the oxysterol production. British journal of pharmacology, 178(16), 3220-3234.

Ali, T., Hannaoui, S., Nemani, S., Tahir, W., Zemlyankina, I., Cherry, P., ... & Gilch, S. (2021). Oral administration of repurposed drug targeting Cyp46A1 increases survival times of prion infected mice. Acta neuropathologica communications, 9(1), 1-14.

Han, M., Wang, S., Yang, N., Wang, X., Zhao, W., Saed, H. S., ... & Wang, J. (2020). Therapeutic implications of altered cholesterol homeostasis mediated by loss of CYP46A1 in human glioblastoma. EMBO molecular medicine, 12(1), e10924.

Petrov, A. M., Lam, M., Mast, N., Moon, J., Li, Y., Maxfield, E., & Pikuleva, I. A. (2019). CYP46A1 activation by efavirenz leads to behavioral improvement without significant changes in amyloid plaque load in the brain of 5XFAD mice. Neurotherapeutics, 16(3), 710-724.

Petrov, A. M., & Pikuleva, I. A. (2019). Cholesterol 24-hydroxylation by CYP46A1: benefits of modulation for brain diseases. Neurotherapeutics, 16(3), 635-648.

Kacher, R., Lamazière, A., Heck, N., Kappes, V., Mounier, C., Despres, G., ... & Betuing, S. (2019). CYP46A1 gene therapy deciphers the role of brain cholesterol metabolism in Huntington’s disease. Brain, 142(8), 2432-2450.

Theofilopoulos, S., de Oliveira, W. A. A., Yang, S., Yutuc, E., Saeed, A., Abdel-Khalik, J., ... & Arenas, E. (2019). 24 (S), 25-Epoxycholesterol and cholesterol 24S-hydroxylase (CYP46A1) overexpression promote midbrain dopaminergic neurogenesis in vivo. Journal of Biological Chemistry, 294(11), 4169-4176.

Mast, N., Verwilst, P., Wilkey, C. J., Guengerich, F. P., & Pikuleva, I. A. (2019). In vitro activation of cytochrome P450 46A1 (CYP46A1) by efavirenz-related compounds. Journal of medicinal chemistry, 63(12), 6477-6488.

Mast, N., Anderson, K. W., Johnson, K. M., Phan, T. T., Guengerich, F. P., & Pikuleva, I. A. (2017). In vitro cytochrome P450 46A1 (CYP46A1) activation by neuroactive compounds. Journal of Biological Chemistry, 292(31), 12934-12946.

Mast, N., Lin, J. B., Anderson, K. W., Bjorkhem, I., & Pikuleva, I. A. (2017). Transcriptional and post-translational changes in the brain of mice deficient in cholesterol removal mediated by cytochrome P450 46A1 (CYP46A1). PLoS One, 12(10), e0187168.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Recombinant Mouse Anti-CYP46A1 Recombinant Antibody (P6E4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare