Rabbit Anti-CYP4X1 Recombinant Antibody (EG912) (CBMAB-EN1083-LY)

The product is antibody recognizes CYP4X1. The antibody EG912 immunoassay techniques such as: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000.
See all CYP4X1 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

EG912

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human Cytochrome P450 4X1.

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cytochrome P450 Family 4 Subfamily X Member 1

Introduction

CYP4X1 (Cytochrome P450 Family 4 Subfamily X Member 1) is a Protein Coding gene. Among its related pathways are superpathway of tryptophan utilization and Cytochrome P450 - arranged by substrate type. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is CYP4Z1.

Entrez Gene ID

260293

UniProt ID

Q8N118

Alternative Names

Cytochrome P450 Family 4 Subfamily X Member 1; Cytochrome P450, Family 4, Subfamily X, Polypeptide 1; CYPIVX1; Cytochrome P450 4X1; EC 1.14.14.1;

Function

A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Topology

Helical: 14-34

References

Chen, Y., Liu, Y., Wang, Y., Chen, X., Wang, C., Chen, X., ... & Zhou, X. (2022). Prevotellaceae produces butyrate to alleviate PD-1/PD-L1 inhibitor-related cardiotoxicity via PPARα-CYP4X1 axis in colonic macrophages. Journal of Experimental & Clinical Cancer Research, 41(1), 1-21.

Idowu, O. A. (2021). Expression, Purification and Characterization of the Human Cytochrome P450 Enzyme 4x1 (CYP4X1) Using Substrate Binding Affinity, Metabolic Assays and Protein Crystallization.

Gebremedhin, D., Guerts, A., & Lombard, J. H. (2020). Role of Nrf2 in Regulation of Expression of CYP 2C11 and CYP 4X1 Epoxygenases and Production of Epoxyeicosatrienoic Acids (EETs) in the Rat Brain. The FASEB Journal, 34(S1), 1-1.

Wang, C., Li, Y., Chen, H., Huang, K., Liu, X., Qiu, M., ... & Yang, J. (2018). CYP4X1 inhibition by flavonoid CH625 normalizes glioma vasculature through reprogramming TAMs via CB2 and EGFR-STAT3 Axis. Journal of Pharmacology and Experimental Therapeutics, 365(1), 72-83.

Poleggi, A., Van Der Lee, S., Capellari, S., Puopolo, M., Ladogana, A., De Pascali, E., ... & Pocchiari, M. (2018). Age at onset of genetic (E200K) and sporadic Creutzfeldt-Jakob diseases is modulated by the CYP4X1 gene. Journal of Neurology, Neurosurgery & Psychiatry, 89(12), 1243-1249.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Rabbit Anti-CYP4X1 Recombinant Antibody (EG912)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: