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Mouse Anti-CYP7A1 Recombinant Antibody (EG914) (CBMAB-EN1085-LY)

The product is antibody recognizes CYP7A1. The antibody EG914 immunoassay techniques such as: ELISA, WB.
See all CYP7A1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
EG914
Antibody Isotype
IgG
Application
ELISA, WB

Basic Information

Immunogen
Recombinant fragment of Human CYP7A1 (C-terminus).
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 7 Subfamily A Member 1
Introduction
CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1) is a Protein Coding gene. Diseases associated with CYP7A1 include Hypercholesterolemia Due To Cholesterol 7Alpha-Hydroxylase Deficiency and Sitosterolemia. Among its related pathways are Farnesoid X Receptor Pathway and Metabolism. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is CYP7B1.
Entrez Gene ID
1581
UniProt ID
P22680
Alternative Names
Cytochrome P450 Family 7 Subfamily A Member 1; Cytochrome P450, Family 7, Subfamily A, Polypeptide 1; Cholesterol 7-Alpha-Hydroxylase; Cytochrome P450 7A1; CYPVII; CYP7; Cytochrome P450, Subfamily VIIA (Cholesterol 7 Alpha-Monooxygenase), Polypeptide 1; Cytochrome P450, Subfamily VIIA Polypeptide 1;
Function
A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols) (PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:2384150, PubMed:21813643).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:2384150, PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:21813643).

Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (PubMed:12077124, PubMed:19965590, PubMed:2384150).

7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol (PubMed:11013305, PubMed:12077124).

Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (PubMed:21813643).
Biological Process
Bile acid and bile salt transport Source: Ensembl
Bile acid biosynthetic process Source: UniProtKB
Bile acid signaling pathway Source: Ensembl
Cellular response to cholesterol Source: UniProtKB
Cellular response to glucose stimulus Source: UniProtKB
Cholesterol catabolic process Source: UniProtKB
Cholesterol homeostasis Source: UniProtKB
Negative regulation of collagen biosynthetic process Source: Ensembl
Negative regulation of fatty acid biosynthetic process Source: Ensembl
Positive regulation of cholesterol biosynthetic process Source: Ensembl
Regulation of bile acid biosynthetic process Source: UniProtKB
Regulation of gene expression Source: Ensembl
Regulation of metabolic process Source: Reactome
Response to ethanol Source: Ensembl
Sterol metabolic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
Topology
Helical: 4-24

Cao, K., Zhang, K., Ma, M., Ma, J., Tian, J., & Jin, Y. (2021). Lactobacillus mediates the expression of NPC1L1, CYP7A1, and ABCG5 genes to regulate cholesterol. Food Science & Nutrition, 9(12), 6882-6891.

Iwanicki, T., Balcerzyk, A., Niemiec, P., Trautsolt, W., Grzeszczak, W., Ochalska-Tyka, A., ... & Żak, I. (2019). The relationship between CYP7A1 polymorphisms, coronary artery disease & serum lipid markers. Biomarkers in Medicine, 13(14), 1199-1208.

Chambers, K. F., Day, P. E., Aboufarrag, H. T., & Kroon, P. A. (2019). Polyphenol effects on cholesterol metabolism via bile acid biosynthesis, CYP7A1: a review. Nutrients, 11(11), 2588.

Duan, Y., Zhang, F., Yuan, W., Wei, Y., Wei, M., Zhou, Y., ... & Wu, X. (2019). Hepatic cholesterol accumulation ascribed to the activation of ileum Fxr-Fgf15 pathway inhibiting hepatic Cyp7a1 in high-fat diet-induced obesity rats. Life sciences, 232, 116638.

Zhang, T., Zhao, M., Lu, D., Wang, S., Yu, F., Guo, L., ... & Wu, B. (2018). REV-ERBα regulates CYP7A1 through repression of liver receptor homolog-1. Drug Metabolism and Disposition, 46(3), 248-258.

Qayyum, F., Lauridsen, B. K., Frikke-Schmidt, R., Kofoed, K. F., Nordestgaard, B. G., & Tybjærg-Hansen, A. (2018). Genetic variants in CYP7A1 and risk of myocardial infarction and symptomatic gallstone disease. European Heart Journal, 39(22), 2106-2116.

Wang, D., Hartmann, K., Seweryn, M., & Sadee, W. (2018). Interactions between regulatory variants in CYP7A1 (cholesterol 7α-hydroxylase) promoter and enhancer regions regulate CYP7A1 expression. Circulation: Genomic and Precision Medicine, 11(10), e002082.

Enya, S., Kawakami, K., Suzuki, Y., & Kawaoka, S. (2018). A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor–liver crosstalk in causing adverse effects on the host. Disease models & mechanisms, 11(8), dmm032383.

Abdullah, M. M., Eck, P. K., Couture, P., Lamarche, B., & Jones, P. J. (2018). The combination of single nucleotide polymorphisms rs6720173 (ABCG5), rs3808607 (CYP7A1), and rs760241 (DHCR7) is associated with differing serum cholesterol responses to dairy consumption. Applied Physiology, Nutrition, and Metabolism, 43(10), 1090-1093.

Wang, Y., Ding, Y., Li, J., Chavan, H., Matye, D., Ni, H. M., ... & Li, T. (2017). Targeting the enterohepatic bile acid signaling induces hepatic autophagy via a CYP7A1–AKT–mTOR axis in mice. Cellular and molecular gastroenterology and hepatology, 3(2), 245-260.

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For research use only. Not intended for any clinical use.

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