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Mouse Anti-DAAM1 Recombinant Antibody (5D3) (CBMAB-D0145-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dishevelled Associated Activator Of Morphogenesis 1 (DAAM1). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DAAM1 antibodies
Published Data
Fig.1 Localization of Daam1 in the endocytic vesicles.
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Fig.2 HA-tagged EphB2 and Myc-tagged Daam1 were coexpressed in HEK293 cells.
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Fig.3 The EphB2 proteins associated with Daam1 were phosphorylated when coprecipitated with Daam1 and detected with an anti-phospho-tyrosine antibody.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
5D3
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
The immunogen sequence: MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV EELDVMFSEL VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT SWPEFYIDQL
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-110

Target

Full Name
dishevelled associated activator of morphogenesis 1
Introduction
DAAM1 contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. DAAM1 is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. DAAM1 also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules.
Entrez Gene ID
23002
UniProt ID
Q9Y4D1
Alternative Names
Dishevelled Associated Activator Of Morphogenesis 1; KIAA0666;
Function
Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed:16630611, PubMed:17482208).

Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity).

Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity).
Biological Process
Actin cytoskeleton organization Source: InterPro
Wnt signaling pathway, planar cell polarity pathway Source: Reactome
Cellular Location
Cilium basal body; Cytoplasm. Perinuclear.

Dai, B., Shen, Y., Yan, T., & Zhang, A. (2020). Wnt5a/ROR1 activates DAAM1 and promotes the migration in osteosarcoma cells. Oncology Reports, 43(2), 601-608.

Mei, J., Xu, B., Hao, L., Xiao, Z., Liu, Y., Yan, T., & Zhu, Y. (2020). Overexpressed DAAM1 correlates with metastasis and predicts poor prognosis in breast cancer. Pathology-Research and Practice, 216(3), 152736.

Mei, J., Yan, T., Huang, Y., Xia, T., Chang, F., Shen, S., ... & Zhu, Y. (2019). A DAAM1 3′-UTR SNP mutation regulates breast cancer metastasis through affecting miR-208a-5p-DAAM1-RhoA axis. Cancer cell international, 19(1), 1-12.

Li, M. Y., Peng, W. H., Wu, C. H., Chang, Y. M., Lin, Y. L., Chang, G. D., ... & Chen, G. C. (2019). PTPN3 suppresses lung cancer cell invasiveness by counteracting Src-mediated DAAM1 activation and actin polymerization. Oncogene, 38(44), 7002-7016.

Xiong, H., Yan, T., Zhang, W., Shi, F., Jiang, X., Wang, X., ... & Zhu, Y. (2018). miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer. Cellular signalling, 44, 33-42.

Liu, G., Yan, T., Li, X., Sun, J., Zhang, B., Wang, H., & Zhu, Y. (2018). Daam1 activates RhoA to regulate Wnt5a‑induced glioblastoma cell invasion. Oncology Reports, 39(2), 465-472.

Yanai, S., Wakayama, M., Nakayama, H., Shinozaki, M., Tsukuma, H., Tochigi, N., ... & Shibuya, K. (2017). Implication of overexpression of dishevelled-associated activator of morphogenesis 1 (Daam-1) for the pathogenesis of human Idiopathic Pulmonary Arterial Hypertension (IPAH). Diagnostic Pathology, 12(1), 1-11.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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