Mouse Anti-DAAM2 Recombinant Antibody (E-1) (CBMAB-D0146-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dishevelled Associated Activator Of Morphogenesis 2 (DAAM2). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all DAAM2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

E-1

Antibody Isotype

IgG

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

dishevelled associated activator of morphogenesis 2

Introduction

DAAM2 is key regulator of the Wnt signaling pathway, which is required for various processes during development, such as dorsal patterning, determination of left/right symmetry or myelination in the central nervous system. DAAM2 acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1). DAAM2 is required for canonical Wnt signaling pathway during patterning in the dorsal spinal cord by promoting the aggregation of Disheveled (Dvl) complexes, thereby clustering and formation of Wnt receptor signalosomes and potentiating Wnt activity.

Entrez Gene ID

23500

UniProt ID

Q86T65

Alternative Names

Dishevelled Associated Activator Of Morphogenesis 2; Disheveled-Associated Activator Of Morphogenesis 2; DJ90A20A.1; KIAA0381;

Function

Key regulator of the Wnt signaling pathway, which is required for various processes during development, such as dorsal patterning, determination of left/right symmetry or myelination in the central nervous system. Acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1). Required for canonical Wnt signaling pathway during patterning in the dorsal spinal cord by promoting the aggregation of Disheveled (Dvl) complexes, thereby clustering and formation of Wnt receptor signalosomes and potentiating Wnt activity. During dorsal patterning of the spinal cord, inhibits oligodendrocytes differentiation via interaction with PIP5K1A. Also regulates non-canonical Wnt signaling pathway. Acts downstream of PITX2 in the developing gut and is required for left/right asymmetry within dorsal mesentery: affects mesenchymal condensation by lengthening cadherin-based junctions through WNT5A and non-canonical Wnt signaling, inducing polarized condensation in the left dorsal mesentery necessary to initiate gut rotation. Together with DAAM1, required for myocardial maturation and sarcomere assembly.

Biological Process

Actin cytoskeleton organization Source: InterPro
Dorsal spinal cord development Source: UniProtKB
Negative regulation of oligodendrocyte differentiation Source: UniProtKB
Positive regulation of canonical Wnt signaling pathway Source: UniProtKB
Regulation of canonical Wnt signaling pathway Source: UniProtKB
Regulation of non-canonical Wnt signaling pathway Source: UniProtKB
Wnt signaling pathway Source: UniProtKB-KW

Cellular Location

Extracellular exosome

References

Zhang, Q., Pan, J., Nie, H., Wang, H., An, F., & Zhan, Q. (2022). Dishevelled-Associated Activator of Morphogenesis 2 (DAAM2) Predicts the Immuno-Hot Phenotype in Pancreatic Adenocarcinoma. Frontiers in molecular biosciences, 9, 750083.

Cristobal, C. D., Wang, C. Y., Zuo, Z., Smith, J. A., Lindeke-Myers, A., Bellen, H. J., & Lee, H. K. (2022). Daam2 regulates myelin structure and the oligodendrocyte actin cytoskeleton through Rac1 and Gelsolin. Journal of Neuroscience, 42(9), 1679-1691.

Qi, C., Alsomali, F., Zhong, J., Harris, R. C., Kon, V., Yang, H., & Fogo, A. B. (2021). Increased dishevelled associated activator of morphogenesis 2, a new podocyte-associated protein, in diabetic nephropathy. Nephrology Dialysis Transplantation, 36(6), 1006-1016.

Chen, A., Liu, Z., Wu, Q., & Li, H. (2021). Disheveled-associated activator of morphogenesis 2 promotes invasion of colorectal cancer by activating PAK1 and promoting MMP7 expression. Genes & Genomics, 43(8), 923-935.

Muhammed, A., & Dean, C. (2021). S48 Investigating the role of dishevelled associated activator of Morphogenesis 2 (Daam2) in lung development.

Sun, S. W., Zhou, M., Chen, L., Wu, J. H., Meng, Z. J., Miao, S. Y., ... & Xiong, X. Z. (2019). Whole exome sequencing identifies a rare variant in DAAM2 as a potential candidate in idiopathic pulmonary ossification. Annals of translational medicine, 7(14).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-DAAM2 Recombinant Antibody (1D8)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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