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Mouse Anti-DAPK2 Recombinant Antibody (V2-612769) (CBMAB-A2082-LY)

The product is antibody recognizes DAPK2. The antibody 1A2 immunoassay techniques such as: WB, ELISA.
See all DAPK2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-612769
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
DAPK2 (AAC35001, 281 a.a. ~ 370 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
death-associated protein kinase 2
Introduction
This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq]
Entrez Gene ID
23604
UniProt ID
Q9UIK4
Alternative Names
DRP-1; MGC119312
Function
Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302).

Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421).
Biological Process
Anoikis Source: UniProtKB
Apoptotic process Source: UniProtKB
Intracellular signal transduction Source: UniProtKB
Positive regulation of apoptotic process Source: GO_Central
Positive regulation of eosinophil chemotaxis Source: UniProtKB
Positive regulation of neutrophil chemotaxis Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Regulation of autophagy Source: UniProtKB
Regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Cellular Location
Cytoplasm; Autophagosome lumen

Jiang, Y., Liu, J., Xu, H., Zhou, X., He, L., & Zhu, C. (2021). DAPK2 activates NF-κB through autophagy-dependent degradation of I-κBα during thyroid cancer development and progression. Annals of Translational Medicine, 9(13).

Ni, X., Wang, Y., Wang, P., Chu, C., Xu, H., Hu, J., ... & Qi, H. (2020). Death associated protein kinase 2 suppresses TB interactions and GC formation. Molecular immunology, 128, 249-257.

Shiloh, R., Bialik, S., & Kimchi, A. (2019). Ser289 phosphorylation activates both DAPK1 and DAPK2 but in response to different intracellular signaling pathways. Cell cycle, 18(11), 1169-1176.

Wang, Y., Liu, L. L., Tian, Y., Chen, Y., Zha, W. H., Li, Y., & Wu, F. J. (2019). Upregulation of DAPK2 ameliorates oxidative damage and apoptosis of placental cells in hypertensive disorder complicating pregnancy by suppressing human placental microvascular endothelial cell autophagy through the mTOR signaling pathway. International journal of biological macromolecules, 121, 488-497.

Zhou, Q., Wang, L., Chen, H., Xu, B., Xu, W., Sheng, Y., & Duan, Y. (2019). 2, 3′, 4, 4′, 5-pentachlorobiphenyl induced autophagy of the thyrocytes via DAPK2/PKD/VPS34 pathway. Archives of Toxicology, 93(6), 1639-1648.

Elbadawy, M., Usui, T., Yamawaki, H., & Sasaki, K. (2018). Novel functions of death-associated protein kinases through mitogen-activated protein kinase-related signals. International journal of molecular sciences, 19(10), 3031.

Humbert, M., Federzoni, E. A., & Tschan, M. P. (2017). Distinct TP73–DAPK2–ATG5 pathway involvement in ATO‐mediated cell death versus ATRA‐mediated autophagy responses in APL. Journal of leukocyte biology, 102(6), 1357-1370.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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