Mouse Anti-DBF4 Recombinant Antibody (CBYCD-163) (CBMAB-D0284-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DBF4 Zinc Finger (DBF4). The antibody can be used for immunoassay techniques, such as WB, IHC-P.
See all DBF4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYCD-163

Antibody Isotype

IgG1

Application

WB, IHC-P

Basic Information

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DBF4 Zinc Finger

Introduction

DBF4 (DBF4 Zinc Finger) is a protein coding gene. Among its related pathways are E2F mediated regulation of DNA replication and CDK-mediated phosphorylation and removal of Cdc6. It has proved that the protein has nucleic acid binding and enzyme activator activity. And a paralog is DBF4B.

Entrez Gene ID

10926

UniProt ID

Q9UBU7

Alternative Names

DBF4 Zinc Finger; Activator Of S Phase Kinase; DBF4-Type Zinc Finger-Containing Protein 1; Zinc Finger, DBF-Type Containing 1; DBF4 Zinc Finger A; Chiffon Homolog A; DBF4A; ZDBF1;

Function

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

Biological Process

DNA replication Source: Reactome
DNA replication initiation Source: Reactome
G1/S transition of mitotic cell cycle Source: ProtInc
Positive regulation of nuclear cell cycle DNA replication Source: GO_Central
Regulation of cell cycle phase transition Source: GO_Central

Cellular Location

Nucleus

PTM

Phosphorylation increases its interaction with PSIP1.

References

Bachant, J., Hoffman, E. A., Caridi, C., Nugent, C. I., & Feng, W. (2022). The yeast Dbf4 Zn2+ finger domain suppresses single-stranded DNA at replication forks initiated from a subset of origins. Current Genetics, 1-13.

Greiwe, J. F., Miller, T. C., Locke, J., Martino, F., Howell, S., Schreiber, A., ... & Costa, A. (2022). Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase. Nature structural & molecular biology, 29(1), 10-20.

Cheng, J., Li, N., Huo, Y., Dang, S., Tye, B. K., Gao, N., & Zhai, Y. (2022). Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase. Nature Communications, 13(1), 1-13.

Torres-Zelada, E. F., Stephenson, R. E., Alpsoy, A., Anderson, B. D., Swanson, S. K., Florens, L., ... & Weake, V. M. (2019). The Drosophila Dbf4 ortholog Chiffon forms a complex with Gcn5 that is necessary for histone acetylation and viability. Journal of cell science, 132(2), jcs214072.

Princz, L. N., Wild, P., Bittmann, J., Aguado, F. J., Blanco, M. G., Matos, J., & Pfander, B. (2017). Dbf4‐dependent kinase and the Rtt107 scaffold promote Mus81‐Mms4 resolvase activation during mitosis. The EMBO journal, 36(5), 664-678.

Fang, D., Lengronne, A., Shi, D. I., Forey, R., Skrzypczak, M., Ginalski, K., ... & Lou, H. (2017). Dbf4 recruitment by forkhead transcription factors defines an upstream rate-limiting step in determining origin firing timing. Genes & development, 31(23-24), 2405-2415.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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