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Mouse Anti-DCLK1 Recombinant Antibody (D-3) (CBMAB-D0356-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Doublecortin Like Kinase 1 (DCLK1). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all DCLK1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D-3
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Doublecortin Like Kinase 1
Introduction
DCLK1 belongs to the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of The protein is independent of its protein kinase activity. The protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.
Entrez Gene ID
Human9201
Mouse13175
Rat83825
UniProt ID
HumanO15075
MouseQ9JLM8
RatO08875
Alternative Names
Doublecortin Like Kinase 1; Doublecortin Domain-Containing Protein 3A; Doublecortin-Like And CAM Kinase-Like 1; Doublecortin-Like Kinase 1; EC 2.7.11.1; DCAMKL1; DCDC3A; Serine/Threonine-Protein Kinase DCLK1;
Function
Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Biological Process
Axon extension Source: Ensembl
Central nervous system development Source: ProtInc
Central nervous system projection neuron axonogenesis Source: Ensembl
Dendrite morphogenesis Source: Ensembl
Endosomal transport Source: UniProtKB
Forebrain development Source: Ensembl
Intracellular signal transduction Source: InterPro
Negative regulation of protein localization to nucleus Source: Ensembl
Nervous system development Source: ProtInc
Neuron migration Source: Ensembl
Neuron projection morphogenesis Source: GO_Central
Peptidyl-serine phosphorylation Source: Ensembl
Protein phosphorylation Source: ProtInc
Response to virus Source: UniProtKB
Cellular Location
Integral component of plasma membrane; Postsynaptic density

Patel, O., Roy, M. J., Kropp, A., Hardy, J. M., Dai, W., & Lucet, I. S. (2021). Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1. Communications biology, 4(1), 1-13.

Agulto, R. L., Rogers, M. M., Tan, T. C., Ramkumar, A., Downing, A. M., Bodin, H., ... & Ori-McKenney, K. M. (2021). Autoregulatory control of microtubule binding in doublecortin-like kinase 1. Elife, 10, e60126.

Onisha, P., Roy, M. J., Ashleigh, K., Hardy, J. M., Weiwen, D., & Lucet, I. S. (2021). Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1. Communications Biology, 4(1).

Ferguson, F. M., Nabet, B., Raghavan, S., Liu, Y., Leggett, A. L., Kuljanin, M., ... & Gray, N. S. (2020). Discovery of a selective inhibitor of doublecortin like kinase 1. Nature chemical biology, 16(6), 635-643.

Vázquez-Vélez, G. E., Gonzales, K. A., Revelli, J. P., Adamski, C. J., Naini, F. A., Bajić, A., ... & Zoghbi, H. Y. (2020). Doublecortin-like kinase 1 regulates α-synuclein levels and toxicity. Journal of Neuroscience, 40(2), 459-477.

Wang, J., Wang, S., Zhou, J., & Qian, Q. (2018). miR-424-5p regulates cell proliferation, migration and invasion by targeting doublecortin-like kinase 1 in basal-like breast cancer. Biomedicine & Pharmacotherapy, 102, 147-152.

Li, J., Wang, Y., Ge, J., Li, W., Yin, L., Zhao, Z., ... & Wang, H. (2018). Doublecortin-like kinase 1 (DCLK1) regulates B cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) and is associated with metastasis and prognosis in pancreatic cancer. Cellular Physiology and Biochemistry, 51(1), 262-277.

Fan, M., Qian, N., & Dai, G. (2017). Expression and prognostic significance of doublecortin‑like kinase 1 in patients with hepatocellular carcinoma. Oncology Letters, 14(6), 7529-7537.

Westphalen, C. B., Quante, M., & Wang, T. C. (2017). Functional implication of Dclk1 and Dclk1-expressing cells in cancer. Small GTPases, 8(3), 164-171.

Wu, X., Ruan, Y., Jiang, H., & Xu, C. (2017). MicroRNA-424 inhibits cell migration, invasion, and epithelial mesenchymal transition by downregulating doublecortin-like kinase 1 in ovarian clear cell carcinoma. The International Journal of Biochemistry & Cell Biology, 85, 66-74.

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For research use only. Not intended for any clinical use.

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