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Rabbit Anti-DDAH1 Monoclonal Antibody (EPR13921) (CBMAB-0991-YC)

Provided herein is a rabbit monoclonal antibody against Human DDAH1. The antibody, clone EPR13921, can be used for immunoassay techniques, such as FC, IHC-P, IP and WB.
See all DDAH1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
EPR13921
Antibody Isotype
IgG
Application
FC, IHC-P, IP, WB

Basic Information

Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDAH1 aa 1-100.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Dimethylarginine Dimethylaminohydrolase 1
Introduction
Dimethylarginine dimethylaminohydrolase 1 (DDAH1) belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
Entrez Gene ID
23576
UniProt ID
O94760
Alternative Names
DDAH; DDAHI; DDAH-1; HEL-S-16
Function
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Biological Process
Arginine catabolic process Source: BHF-UCL
Arginine metabolic process Source: GO_Central
Citrulline metabolic process Source: UniProtKB
Negative regulation of cell population proliferation Source: BHF-UCL
Negative regulation of cellular response to hypoxia Source: BHF-UCL
Negative regulation of vascular permeability Source: BHF-UCL
Nitric oxide mediated signal transduction Source: ProtInc
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of nitric oxide biosynthetic process Source: BHF-UCL
Regulation of nitric-oxide synthase activity Source: Reactome
Regulation of systemic arterial blood pressure Source: BHF-UCL
Cellular Location
Cytosol; Extracellular exosome

Xie, Z., Hou, L., Shen, S., Wu, Y., Wang, J., Jie, Z., ... & Fan, S. (2022). Mechanical force promotes dimethylarginine dimethylaminohydrolase 1-mediated hydrolysis of the metabolite asymmetric dimethylarginine to enhance bone formation. Nature communications, 13(1), 1-15.

Kopaliani, I., Jarzebska, N., Billoff, S., Kolouschek, A., Martens-Lobenhoffer, J., Bornstein, S. R., ... & Rodionov, R. N. (2021). Overexpression of dimethylarginine dimethylaminohydrolase 1 protects from angiotensin II-induced cardiac hypertrophy and vascular remodeling. American Journal of Physiology-Heart and Circulatory Physiology, 321(5), H825-H838.

Wang, D., Li, H., Weir, E. K., Xu, Y., Xu, D., & Chen, Y. (2019). Dimethylarginine dimethylaminohydrolase 1 deficiency aggravates monocrotaline-induced pulmonary oxidative stress, pulmonary arterial hypertension and right heart failure in rats. International Journal of Cardiology, 295, 14-20.

Wang, Y., Wang, E., Zhang, Y., Madamsetty, V. S., Ji, B., Radisky, D. C., ... & Mukhopadhyay, D. (2019). Neuropilin‐1 maintains dimethylarginine dimethylaminohydrolase 1 expression in endothelial cells, and contributes to protection from angiotensin II–induced hypertension. The FASEB Journal, 33(1), 494-500.

Reddy, K. R. K., Dasari, C., Duscharla, D., Supriya, B., Ram, N. S., Surekha, M. V., ... & Ummanni, R. (2018). Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA). Angiogenesis, 21(1), 79-94.

Buijs, N., Oosterink, J. E., Jessup, M., Schierbeek, H., Stolz, D. B., Houdijk, A. P., ... & van Leeuwen, P. A. (2017). A new key player in VEGF-dependent angiogenesis in human hepatocellular carcinoma: dimethylarginine dimethylaminohydrolase 1. Angiogenesis, 20(4), 557-565.

Xu, X., Zhang, P., Kwak, D., Fassett, J., Yue, W., Atzler, D., ... & Chen, Y. (2017). Cardiomyocyte dimethylarginine dimethylaminohydrolase-1 (DDAH1) plays an important role in attenuating ventricular hypertrophy and dysfunction. Basic research in cardiology, 112(5), 1-11.

Hulin, J. A., Tommasi, S., Elliot, D., Hu, D. G., Lewis, B. C., & Mangoni, A. A. (2017). MiR-193b regulates breast cancer cell migration and vasculogenic mimicry by targeting dimethylarginine dimethylaminohydrolase 1. Scientific reports, 7(1), 1-15.

Li, T., Feng, R., Zhao, C., Wang, Y., Wang, J., Liu, S., ... & Lu, Z. (2017). Dimethylarginine dimethylaminohydrolase 1 protects against high-fat diet-induced hepatic steatosis and insulin resistance in mice. Antioxidants & Redox Signaling, 26(11), 598-609.

Shi, L., Zhao, C., Wang, H., Lei, T., Liu, S., Cao, J., & Lu, Z. (2017). Dimethylarginine dimethylaminohydrolase 1 deficiency induces the epithelial to mesenchymal transition in renal proximal tubular epithelial cells and exacerbates kidney damage in aged and diabetic mice. Antioxidants & Redox Signaling, 27(16), 1347-1360.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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