Sign in or Register   Sign in or Register
  |  

Mouse Anti-DDX6 Recombinant Antibody (10B2681) (CBMAB-D0628-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DEAD-Box Helicase 6 (DDX6). The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all DDX6 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
10B2681
Antibody Isotype
IgG1, κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Recombinant protein corresponding to a portion of amino acids within a.a.1-484 of human DDX6 with a GST tag (26kD)
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DEAD-Box Helicase 6
Introduction
DDX6 belongs to the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Entrez Gene ID
1656
UniProt ID
P26196
Alternative Names
DEAD-Box Helicase 6; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 6 (RNA Helicase, 54kD); DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6; DEAD (Asp-Glu-Ala-Asp) Box Helicase 6; ATP-Dependent RNA Helicase P54; DEAD Box Protein 6; Oncogene RCK; HLR2;
Function
Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed:25995375, PubMed:27342281, PubMed:31422817).

Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:27342281).

In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915).

Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).
Biological Process
Exonucleolytic catabolism of deadenylated mRNA Source: Reactome
Negative regulation of neuron differentiation Source: Ensembl
Negative regulation of translation Source: UniProtKB
P-body assembly Source: UniProtKB
Positive regulation of miRNA mediated inhibition of translation Source: UniProtKB
Spermatid differentiation Source: Ensembl
Stem cell population maintenance Source: Ensembl
Stress granule assembly Source: GO_Central
Viral RNA genome packaging Source: CACAO
Cellular Location
Nucleus; Cytoplasm; P-body. Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:28216671). Upon cellular stress, relocalizes to stress granules (PubMed:26184334).
Involvement in disease
Intellectual developmental disorder with impaired language and dysmorphic facies (IDDILF):
An autosomal dominant disorder characterized by intellectual disability, developmental delay, impaired language development, and dysmorphic features including telecanthus, epicanthus, arched eyebrows and low-set ears. Additional features include feeding difficulties, mild cardiac or genitourinary defects, and distal skeletal anomalies.
A chromosomal aberration involving DDX6 may be a cause of hematopoietic tumors such as B-cell lymphomas. Translocation t(11;14)(q23;q32).
PTM
Sumoylated (PubMed:26184334).

Zhao, Z., Wu, C., He, X., Zhao, E., Hu, S., Han, Y., ... & Huang, S. (2021). miR-152-3p aggravates vascular endothelial cell dysfunction by targeting DEAD-box helicase 6 (DDX6) under hypoxia. Bioengineered, 12(1), 4899-4910.

Balak, C., Bénard, M., Schaefer, E., Iqbal, S., Ramsey, K., Ernoult-Lange, M., ... & Piton, A. (2019). Rare de novo missense variants in RNA helicase DDX6 cause intellectual disability and dysmorphic features and lead to P-Body defects and RNA dysregulation. The American Journal of Human Genetics, 105(3), 509-525.

Taniguchi, K., Iwatsuki, A., Sugito, N., Shinohara, H., Kuranaga, Y., Oshikawa, Y., ... & Akao, Y. (2018). Oncogene RNA helicase DDX6 promotes the process of c‐Myc expression in gastric cancer cells. Molecular Carcinogenesis, 57(5), 579-589.

Zhen, Z., Zhang, M., Yuan, X., Qu, B., Yu, Y., Gao, X., & Qiu, Y. (2018). DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells. In Vitro Cellular & Developmental Biology-Animal, 54(1), 52-60.

Tajirika, T., Tokumaru, Y., Taniguchi, K., Sugito, N., Matsuhashi, N., Futamura, M., ... & Yoshida, K. (2018). DEAD-box protein RNA-helicase DDX6 regulates the expression of HER2 and FGFR2 at the post-transcriptional step in gastric cancer cells. International Journal of Molecular Sciences, 19(7), 2005.

Núñez, R. D., Budt, M., Saenger, S., Paki, K., Arnold, U., Sadewasser, A., & Wolff, T. (2018). The RNA helicase DDX6 associates with RIG-I to augment induction of antiviral signaling. International journal of molecular sciences, 19(7), 1877.

Biegel, J. M., Henderson, E., Cox, E. M., Bonenfant, G., Netzband, R., Kahn, S., ... & Pager, C. T. (2017). Cellular DEAD-box RNA helicase DDX6 modulates interaction of miR-122 with the 5′ untranslated region of hepatitis C virus RNA. Virology, 507, 231-241.

Lumb, J. H., Li, Q., Popov, L. M., Ding, S., Keith, M. T., Merrill, B. D., ... & Carette, J. E. (2017). DDX6 represses aberrant activation of interferon-stimulated genes. Cell reports, 20(4), 819-831.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-DDX6 Recombinant Antibody (10B2681)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare