Rabbit Anti-DDX6 Recombinant Antibody (D0632) (CBMAB-D0632-YC)

Name: Rabbit Anti-DDX6 Recombinant Antibody (D0632)
SKU: CBMAB-D0632-YC
Rating: 5 (1 reviews)

See all DDX6 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D0632

Application

WB, IHC-P

Immunogen

Recombinant protein within human ddx6 (aa 81-220).

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:1,000
IHC-P	1:50-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & TBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

DEAD-Box Helicase 6

Introduction

DDX6 belongs to the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Entrez Gene ID

Human	1656
Mouse	13209
Rat	500988

UniProt ID

Human	P26196
Mouse	P54823
Rat	D3ZD73

Alternative Names

DEAD-Box Helicase 6; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 6 (RNA Helicase, 54kD); DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6; DEAD (Asp-Glu-Ala-Asp) Box Helicase 6; ATP-Dependent RNA Helicase P54; DEAD Box Protein 6; Oncogene RCK; HLR2;

Function

Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed:25995375, PubMed:27342281, PubMed:31422817).

Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:27342281).

In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915).

Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).

Biological Process

Exonucleolytic catabolism of deadenylated mRNA Source: Reactome
Negative regulation of neuron differentiation Source: Ensembl
Negative regulation of translation Source: UniProtKB
P-body assembly Source: UniProtKB
Positive regulation of miRNA mediated inhibition of translation Source: UniProtKB
Spermatid differentiation Source: Ensembl
Stem cell population maintenance Source: Ensembl
Stress granule assembly Source: GO_Central
Viral RNA genome packaging Source: CACAO

Cellular Location

Nucleus; Cytoplasm; P-body. Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:28216671). Upon cellular stress, relocalizes to stress granules (PubMed:26184334).

Involvement in disease

Intellectual developmental disorder with impaired language and dysmorphic facies (IDDILF):
An autosomal dominant disorder characterized by intellectual disability, developmental delay, impaired language development, and dysmorphic features including telecanthus, epicanthus, arched eyebrows and low-set ears. Additional features include feeding difficulties, mild cardiac or genitourinary defects, and distal skeletal anomalies.
A chromosomal aberration involving DDX6 may be a cause of hematopoietic tumors such as B-cell lymphomas. Translocation t(11;14)(q23;q32).

PTM

Sumoylated (PubMed:26184334).

Zhao, Z., Wu, C., He, X., Zhao, E., Hu, S., Han, Y., ... & Huang, S. (2021). miR-152-3p aggravates vascular endothelial cell dysfunction by targeting DEAD-box helicase 6 (DDX6) under hypoxia. Bioengineered, 12(1), 4899-4910.

Balak, C., Bénard, M., Schaefer, E., Iqbal, S., Ramsey, K., Ernoult-Lange, M., ... & Piton, A. (2019). Rare de novo missense variants in RNA helicase DDX6 cause intellectual disability and dysmorphic features and lead to P-Body defects and RNA dysregulation. The American Journal of Human Genetics, 105(3), 509-525.

Taniguchi, K., Iwatsuki, A., Sugito, N., Shinohara, H., Kuranaga, Y., Oshikawa, Y., ... & Akao, Y. (2018). Oncogene RNA helicase DDX6 promotes the process of c‐Myc expression in gastric cancer cells. Molecular Carcinogenesis, 57(5), 579-589.

Zhen, Z., Zhang, M., Yuan, X., Qu, B., Yu, Y., Gao, X., & Qiu, Y. (2018). DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells. In Vitro Cellular & Developmental Biology-Animal, 54(1), 52-60.

Tajirika, T., Tokumaru, Y., Taniguchi, K., Sugito, N., Matsuhashi, N., Futamura, M., ... & Yoshida, K. (2018). DEAD-box protein RNA-helicase DDX6 regulates the expression of HER2 and FGFR2 at the post-transcriptional step in gastric cancer cells. International Journal of Molecular Sciences, 19(7), 2005.

Núñez, R. D., Budt, M., Saenger, S., Paki, K., Arnold, U., Sadewasser, A., & Wolff, T. (2018). The RNA helicase DDX6 associates with RIG-I to augment induction of antiviral signaling. International journal of molecular sciences, 19(7), 1877.

Biegel, J. M., Henderson, E., Cox, E. M., Bonenfant, G., Netzband, R., Kahn, S., ... & Pager, C. T. (2017). Cellular DEAD-box RNA helicase DDX6 modulates interaction of miR-122 with the 5′ untranslated region of hepatitis C virus RNA. Virology, 507, 231-241.

Lumb, J. H., Li, Q., Popov, L. M., Ding, S., Keith, M. T., Merrill, B. D., ... & Carette, J. E. (2017). DDX6 represses aberrant activation of interferon-stimulated genes. Cell reports, 20(4), 819-831.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-DDX6 Recombinant Antibody (D0632)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-DDX6 Recombinant Antibody (3D2) (CAT#: V2LY-0425-LY121)

Mouse Anti-DDX6 Recombinant Antibody (D0634) (CAT#: V2LY-0425-LY123)

Mouse Anti-DDX6 Recombinant Antibody (D0630) (CAT#: V2LY-0425-LY120)

Mouse Anti-DDX59 Recombinant Antibody (D0628) (CAT#: V2LY-0425-LY118)

Mouse Anti-DDX6 Recombinant Antibody (D0629) (CAT#: V2LY-0425-LY119)

Mouse Anti-DDX6 Recombinant Antibody (D0635) (CAT#: CBMAB-D0635-YC)

Rabbit Anti-DDX6 Recombinant Antibody (
6D8) (CAT#: V2LY-0725-LY1010)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry