Rabbit Anti-DDX6 Recombinant Antibody (
6D8) (V2LY-0725-LY1010)

Name: Rabbit Anti-DDX6 Recombinant Antibody (6D8)
SKU: V2LY-0725-LY1010
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-DDX6 Recombinant Antibody (6D8) (V2LY-0725-LY1010) in IHC

IHC image of V2LY-0725-LY1010 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

6D8

Application

ELISA, IHC

Immunogen

A synthesized peptide derived from human DDX6.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DEAD-Box Helicase 6

Entrez Gene ID

1656

UniProt ID

P26196

Function

Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed:25995375, PubMed:27342281, PubMed:31422817).

Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:27342281).

In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915).

Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).

Biological Process

Exonucleolytic catabolism of deadenylated mRNA Source: Reactome
Negative regulation of neuron differentiation Source: Ensembl
Negative regulation of translation Source: UniProtKB
P-body assembly Source: UniProtKB
Positive regulation of miRNA mediated inhibition of translation Source: UniProtKB
Spermatid differentiation Source: Ensembl
Stem cell population maintenance Source: Ensembl
Stress granule assembly Source: GO_Central
Viral RNA genome packaging Source: CACAO

Cellular Location

Nucleus; Cytoplasm; P-body. Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:28216671). Upon cellular stress, relocalizes to stress granules (PubMed:26184334).

Involvement in disease

Intellectual developmental disorder with impaired language and dysmorphic facies (IDDILF):
An autosomal dominant disorder characterized by intellectual disability, developmental delay, impaired language development, and dysmorphic features including telecanthus, epicanthus, arched eyebrows and low-set ears. Additional features include feeding difficulties, mild cardiac or genitourinary defects, and distal skeletal anomalies.
A chromosomal aberration involving DDX6 may be a cause of hematopoietic tumors such as B-cell lymphomas. Translocation t(11;14)(q23;q32).

PTM

Sumoylated (PubMed:26184334).

Zhao, Z., Wu, C., He, X., Zhao, E., Hu, S., Han, Y., ... & Huang, S. (2021). miR-152-3p aggravates vascular endothelial cell dysfunction by targeting DEAD-box helicase 6 (DDX6) under hypoxia. Bioengineered, 12(1), 4899-4910.

Balak, C., Bénard, M., Schaefer, E., Iqbal, S., Ramsey, K., Ernoult-Lange, M., ... & Piton, A. (2019). Rare de novo missense variants in RNA helicase DDX6 cause intellectual disability and dysmorphic features and lead to P-Body defects and RNA dysregulation. The American Journal of Human Genetics, 105(3), 509-525.

Taniguchi, K., Iwatsuki, A., Sugito, N., Shinohara, H., Kuranaga, Y., Oshikawa, Y., ... & Akao, Y. (2018). Oncogene RNA helicase DDX6 promotes the process of c‐Myc expression in gastric cancer cells. Molecular Carcinogenesis, 57(5), 579-589.

Zhen, Z., Zhang, M., Yuan, X., Qu, B., Yu, Y., Gao, X., & Qiu, Y. (2018). DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells. In Vitro Cellular & Developmental Biology-Animal, 54(1), 52-60.

Tajirika, T., Tokumaru, Y., Taniguchi, K., Sugito, N., Matsuhashi, N., Futamura, M., ... & Yoshida, K. (2018). DEAD-box protein RNA-helicase DDX6 regulates the expression of HER2 and FGFR2 at the post-transcriptional step in gastric cancer cells. International Journal of Molecular Sciences, 19(7), 2005.

Núñez, R. D., Budt, M., Saenger, S., Paki, K., Arnold, U., Sadewasser, A., & Wolff, T. (2018). The RNA helicase DDX6 associates with RIG-I to augment induction of antiviral signaling. International journal of molecular sciences, 19(7), 1877.

Biegel, J. M., Henderson, E., Cox, E. M., Bonenfant, G., Netzband, R., Kahn, S., ... & Pager, C. T. (2017). Cellular DEAD-box RNA helicase DDX6 modulates interaction of miR-122 with the 5′ untranslated region of hepatitis C virus RNA. Virology, 507, 231-241.

Lumb, J. H., Li, Q., Popov, L. M., Ding, S., Keith, M. T., Merrill, B. D., ... & Carette, J. E. (2017). DDX6 represses aberrant activation of interferon-stimulated genes. Cell reports, 20(4), 819-831.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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