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Rabbit Anti-DLX5 Monoclonal Antibody (EPR4488) (CBMAB-1031-YC)

Provided herein is a rabbit monoclonal antibody against Human DLX5. The antibody, clone EPR4488, can be used for immunoassay techniques, such as ICC, IF, IHC-P, IP and WB.
See all DLX5 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EPR4488
Antibody Isotype
IgG
Application
ICC, IF, IHC-P, IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to Human Dlx5 aa 150-250.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
distal-less homeobox 5
Introduction
DLX5 is a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene, which may play a role in bone development and fracture healing.
Entrez Gene ID
Human1749
Mouse13395
Rat25431
UniProt ID
HumanP56178
MouseP70396
RatP50575
Alternative Names
SHFM1D
Function
Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.
Biological Process
Anatomical structure formation involved in morphogenesis Source: GO_Central
BMP signaling pathway Source: BHF-UCL
Cell differentiation Source: GO_Central
Cell population proliferation Source: UniProtKB
Cellular response to BMP stimulus Source: BHF-UCL
Embryonic limb morphogenesis Source: Ensembl
Endochondral ossification Source: UniProtKB
Epithelial cell differentiation Source: Ensembl
Face morphogenesis Source: Ensembl
Inner ear morphogenesis Source: Ensembl
Interneuron axon guidance Source: Ensembl
Nervous system development Source: ProtInc
Olfactory bulb interneuron differentiation Source: Ensembl
Olfactory pit development Source: Ensembl
Osteoblast differentiation Source: UniProtKB
Positive regulation of canonical Wnt signaling pathway Source: Ensembl
Positive regulation of epithelial cell proliferation Source: Ensembl
Positive regulation of gene expression Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Roof of mouth development Source: Ensembl
Skeletal system development Source: ProtInc
Cellular Location
Nucleus
Involvement in disease
Split-hand/foot malformation 1 with sensorineural hearing loss, autosomal recessive (SHFM1D):
A disease characterized by the association of split-hand/foot malformation with deafness. Split-hand/foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting.
PTM
Phosphorylated. Phosphorylation of Ser-34 and Ser-217 by MAPK14 enhances its transcriptional activity. Phosphorylation by CaMK2 increases its protein stability (By similarity).

Mullen, R. D., Bellessort, B., Levi, G., & Behringer, R. D. (2022). Distal-less homeobox genes Dlx5/6 regulate Mulllerian duct regression. bioRxiv.

Kim, C. Y., Cho, D. H., Chung, D. J., Lee, S. H., Han, Y., & Lee, K. Y. (2021). Dlx5 Represses the Transcriptional Activity of PPARγ. Biological and Pharmaceutical Bulletin, 44(9), 1303-1308.

Zhang, G., Zhou, Y., Su, M., Yang, X., & Zeng, B. (2020). Inhibition of microRNA‐27b‐3p relieves osteoarthritis pain via regulation of KDM4B‐dependent DLX5. Biofactors, 46(5), 788-802.

Sun, S., Yang, F., Zhu, Y., & Zhang, S. (2020). KDM4A promotes the growth of non-small cell lung cancer by mediating the expression of Myc via DLX5 through the Wnt/β-catenin signaling pathway. Life Sciences, 262, 118508.

Yang, H., Fan, J., Cao, Y., Gao, R., & Fan, Z. (2019). Distal-less homeobox 5 promotes the osteo-/dentinogenic differentiation potential of stem cells from apical papilla by activating histone demethylase KDM4B through a positive feedback mechanism. Experimental Cell Research, 374(1), 221-230.

Yang, D., Okamura, H., & Qiu, L. (2018). Upregulated osterix expression elicited by Runx2 and Dlx5 is required for the accelerated osteoblast differentiation in PP2A Cα‐knockdown cells. Cell Biology International, 42(4), 403-410.

Lee, K. M., Park, K. H., Hwang, J. S., Lee, M., Yoon, D. S., Ryu, H. A., ... & Lee, J. W. (2018). Inhibition of STAT5A promotes osteogenesis by DLX5 regulation. Cell death & disease, 9(11), 1-13.

Lee, H. L., Qadir, A. S., Park, H. J., Chung, E., Lee, Y. S., Woo, K. M., ... & Baek, J. H. (2018). cAMP/protein kinase A signaling inhibits Dlx5 expression via activation of CREB and subsequent C/EBPβ induction in 3T3-L1 preadipocytes. International Journal of Molecular Sciences, 19(10), 3161.

Heo, J. S., Lee, S. G., & Kim, H. O. (2017). Distal-less homeobox 5 is a master regulator of the osteogenesis of human mesenchymal stem cells. International journal of molecular medicine, 40(5), 1486-1494.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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