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Mouse Anti-DOCK2 Recombinant Antibody (2F4) (CBMAB-D1475-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dedicator Of Cytokinesis 2 (DOCK2). The antibody can be used for immunoassay techniques, such as ELISA.
See all DOCK2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2F4
Antibody Isotype
IgG2a, κ
Application
ELISA

Basic Information

Immunogen
DOCK2 (AAH16996, AA 1-336 full-length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
dedicator of cytokinesis 2
Introduction
DOCK2 belongs to the CDM protein family. It is specifically expressed in hematopoietic cells and is predominantly expressed in peripheral blood leukocytes. The protein is involved in remodeling of the actin cytoskeleton required for lymphocyte migration in response to chemokine signaling. It activates members of the Rho family of GTPases, for example RAC1 and RAC2, by acting as a guanine nucleotide exchange factor (GEF) to exchange bound GDP for free GTP.
Entrez Gene ID
1794
UniProt ID
Q92608
Alternative Names
Dedicator Of Cytokinesis 2; Dedicator Of Cyto-Kinesis 2; Dedicator Of Cytokinesis Protein 2; KIAA0209; IMD40;
Function
Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.
Biological Process
Actin cytoskeleton organization Source: UniProtKB
Alpha-beta T cell proliferation Source: Ensembl
Chemotaxis Source: Ensembl
Establishment of T cell polarity Source: Ensembl
Immunological synapse formation Source: Ensembl
Macropinocytosis Source: UniProtKB
Membrane raft polarization Source: Ensembl
Myeloid dendritic cell activation involved in immune response Source: UniProtKB
Negative thymic T cell selection Source: Ensembl
Positive regulation of phagocytosis Source: UniProtKB
Positive thymic T cell selection Source: Ensembl
Regulation of small GTPase mediated signal transduction Source: Reactome
Small GTPase mediated signal transduction Source: InterPro
Cellular Location
Cytoskeleton; Endomembrane system. Colocalizes with F-actin.
Involvement in disease
Immunodeficiency 40 (IMD40):
A form of combined immunodeficiency characterized by lymphopenia, and defective T-cell, B-cell, and NK-cell responses. Patients suffer from severe invasive bacterial and viral infections in early childhood and may die without bone marrow transplantation.

Qian, G., Adeyanju, O., Roy, S., Sunil, C., Jeffers, A., Guo, X., ... & Tucker, T. A. (2022). DOCK2 promotes pleural fibrosis by modulating mesothelial to mesenchymal transition. American journal of respiratory cell and molecular biology, 66(2), 171-182.

Xu, X., Su, Y., Wu, K., Pan, F., & Wang, A. (2021). DOCK2 contributes to endotoxemia-induced acute lung injury in mice by activating proinflammatory macrophages. Biochemical Pharmacology, 184, 114399.

Chang, L., Yang, J., Jo, C. H., Boland, A., Zhang, Z., McLaughlin, S. H., ... & Barford, D. (2020). Structure of the DOCK2− ELMO1 complex provides insights into regulation of the auto-inhibited state. Nature communications, 11(1), 1-17.

Bjerre, M. T., Strand, S. H., Nørgaard, M., Kristensen, H., Rasmussen, A. K., Mortensen, M. M., ... & Sørensen, K. D. (2019). Aberrant DOCK2, GRASP, HIF3A and PKFP hypermethylation has potential as a prognostic biomarker for prostate cancer. International journal of molecular sciences, 20(5), 1173.

Moens, L., Gouwy, M., Bosch, B., Pastukhov, O., Nieto-Patlàn, A., Siler, U., ... & Meyts, I. (2019). Human DOCK2 deficiency: report of a novel mutation and evidence for neutrophil dysfunction. Journal of clinical immunology, 39(3), 298-308.

Hu, N., Pang, Y., Zhao, H., Si, C., Ding, H., Chen, L., ... & Fu, L. (2019). High expression of DOCK2 indicates good prognosis in acute myeloid leukemia. Journal of Cancer, 10(24), 6088.

Chen, Y., Meng, F., Wang, B., He, L., Liu, Y., & Liu, Z. (2018). Dock2 in the development of inflammation and cancer. European journal of immunology, 48(6), 915-922.

Alizadeh, Z., Mazinani, M., Shakerian, L., Nabavi, M., & Fazlollahi, M. R. (2018). DOCK2 deficiency in a patient with hyper IgM phenotype. Journal of Clinical Immunology, 38(1), 10-12.

Hasan, M. K., Yu, J., Widhopf, G. F., Rassenti, L. Z., Chen, L., Shen, Z., ... & Kipps, T. J. (2018). Wnt5a induces ROR1 to recruit DOCK2 to activate Rac1/2 in chronic lymphocytic leukemia. Blood, The Journal of the American Society of Hematology, 132(2), 170-178.

Sakurai, T., Uruno, T., Sugiura, Y., Tatsuguchi, T., Yamamura, K., Ushijima, M., ... & Fukui, Y. (2018). Cholesterol sulfate is a DOCK2 inhibitor that mediates tissue-specific immune evasion in the eye. Science signaling, 11(541), eaao4874.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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