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Rabbit Anti-E2F2 Recombinant Antibody (EG1019) (CBMAB-EN1202-LY)

The product is antibody recognizes E2F2. The antibody EG1019 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:5000.
See all E2F2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
EG1019
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:5000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human E2F2.
Specificity
Human, Mouse
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
E2F transcription factor 2
Introduction
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.
Entrez Gene ID
Human1870
Mouse242705
UniProt ID
HumanQ14209
MouseP56931
Alternative Names
E2F-2
Research Area
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
Biological Process
Cell cycle Source: UniProtKB-KW
Intrinsic apoptotic signaling pathway by p53 class mediator Source: Ensembl
Lens fiber cell apoptotic process Source: Ensembl
Negative regulation of sprouting angiogenesis Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of cell cycle Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Transcription initiation from RNA polymerase II promoter Source: ProtInc
Cellular Location
Nucleus
PTM
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.

Li, P., Xu, H., Yang, L., Zhan, M., Shi, Y., Zhang, C., ... & Wang, Z. (2022). E2F transcription factor 2-activated DLEU2 contributes to prostate tumorigenesis by upregulating serum and glucocorticoid-induced protein kinase 1. Cell death & disease, 13(1), 1-12.

Lv, H., Yu, J., Zhang, H., Qian, X., Wang, Q., Lu, B., & Sun, Y. (2022). MicroRNA-631 deriving from bone marrow mesenchymal stem cell exosomes facilitates the malignant behavior of non-small cell lung cancer via modulating the E2F family of transcription factor 2/phosphatidylinositol 3‐kinase/Akt signaling pathway. Bioengineered, 13(4), 8382-8395.

Xu, L., Wang, L., Shi, Y., Deng, Y., Oates, J. C., Kamen, D. L., ... & Tsao, B. P. (2022). Up‐Regulated Interleukin‐10 Induced by E2F Transcription Factor 2–MicroRNA‐17‐5p Circuitry in Extrafollicular Effector B Cells Contributes to Autoantibody Production in Systemic Lupus Erythematosus. Arthritis & Rheumatology, 74(3), 496-507.

Li, L., Zhang, Y., Wang, L., & Pan, J. (2021). ChIP-sequencing analysis of E2F transcription factor 2 reveals its role in various biological processes of rheumatoid arthritis synovial fibroblasts. BioScience Trends, 15(2), 132-134.

Li, L., Wang, S., Zhang, Y., & Pan, J. (2021). The E2F transcription factor 2: What do we know?. BioScience Trends.

Mamoor, S. (2021). Differential expression of E2F transcription factor 2 in human epithelial ovarian cancer.

Zhang, R., Wang, L., Pan, J. H., & Han, J. (2018). A critical role of E2F transcription factor 2 in proinflammatory cytokines-dependent proliferation and invasiveness of fibroblast-like synoviocytes in rheumatoid Arthritis. Scientific reports, 8(1), 1-10.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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