Mouse Anti-EDF1 Recombinant Antibody (3E6) (CBMAB-A2448-LY)

Name: Mouse Anti-EDF1 Recombinant Antibody (3E6)
SKU: CBMAB-A2448-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3E6

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

EDF1 (AAH15500.1, 1 a.a. ~ 148 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

EDF1

Introduction

This gene encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene. [provided by RefSeq]

Entrez Gene ID

8721

UniProt ID

O60869

Alternative Names

EDF-1; MBF1; MGC9058

Research Area

Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.

Biological Process

Endothelial cell differentiation Source: UniProtKB
Multicellular organism development Source: ProtInc
Positive regulation of DNA binding Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of lipid metabolic process Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm. Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.

PTM

Phosphorylated (by PKA and PKC).

More Infomation

References

Locatelli, L., Fedele, G., Castiglioni, S., & Maier, J. A. (2021). Magnesium deficiency induces lipid accumulation in vascular endothelial cells via oxidative stress—the potential contribution of edf-1 and pparγ. International Journal of Molecular Sciences, 22(3), 1050.

Feng, F., Wang, J., Bao, R., Li, L., Tong, X., Han, S., ... & Zhang, C. (2020). LncPrep+ 96kb 2.2 kb inhibits estradiol secretion from granulosa cells by inducing EDF1 translocation. Frontiers in Cell and Developmental Biology, 8, 481.

Sinha, N. K., Ordureau, A., Best, K., Saba, J. A., Zinshteyn, B., Sundaramoorthy, E., ... & Green, R. (2020). EDF1 coordinates cellular responses to ribosome collisions. Elife, 9, e58828.

Cazzaniga, A., Locatelli, L., Castiglioni, S., & Maier, J. (2019). The Contribution of EDF1 to PPAR7 Transcriptional Activation in VEGF-Treated Human Endothelial Cells. PPARs in Cellular and Whole Body Energy Metabolism, 19, 93.

Cazzaniga, A., Locatelli, L., Castiglioni, S., & Maier, J. (2018). The contribution of EDF1 to PPARγ transcriptional activation in VEGF-treated human endothelial cells. International journal of molecular sciences, 19(7), 1830.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-EDF1 (AA 1-148) Recombinant Antibody (CBFYE-0437)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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