Mouse Anti-EDF1 Recombinant Antibody (CBFYE-0437) (CBMAB-E0758-FY)

Name: Mouse Anti-EDF1 Recombinant Antibody (CBFYE-0437)
SKU: CBMAB-E0758-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0437

Application

Immunogen

Full-length recombinant corresponding to aa1-148 from EDF1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-148

More Infomation

Target

Full Name

EDF1

Introduction

This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators.

Entrez Gene ID

8721

UniProt ID

O60869

Alternative Names

MBF1; EDF-1; CFAP280

Research Area

Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.

Biological Process

Endothelial cell differentiation Source: UniProtKB
Multicellular organism development Source: ProtInc
Positive regulation of DNA binding Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of lipid metabolic process Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm. Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.

PTM

Phosphorylated (by PKA and PKC).

Locatelli, L., Fedele, G., Castiglioni, S., & Maier, J. A. (2021). Magnesium deficiency induces lipid accumulation in vascular endothelial cells via oxidative stress—the potential contribution of edf-1 and pparγ. International Journal of Molecular Sciences, 22(3), 1050.

Feng, F., Wang, J., Bao, R., Li, L., Tong, X., Han, S., ... & Zhang, C. (2020). LncPrep+ 96kb 2.2 kb inhibits estradiol secretion from granulosa cells by inducing EDF1 translocation. Frontiers in Cell and Developmental Biology, 8, 481.

Sinha, N. K., Ordureau, A., Best, K., Saba, J. A., Zinshteyn, B., Sundaramoorthy, E., ... & Green, R. (2020). EDF1 coordinates cellular responses to ribosome collisions. Elife, 9, e58828.

Cazzaniga, A., Locatelli, L., Castiglioni, S., & Maier, J. (2019). The Contribution of EDF1 to PPAR7 Transcriptional Activation in VEGF-Treated Human Endothelial Cells. PPARs in Cellular and Whole Body Energy Metabolism, 19, 93.

Cazzaniga, A., Locatelli, L., Castiglioni, S., & Maier, J. (2018). The contribution of EDF1 to PPARγ transcriptional activation in VEGF-treated human endothelial cells. International journal of molecular sciences, 19(7), 1830.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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