Rabbit Anti-EIF3K Recombinant Antibody (EG1068) (CBMAB-EN1259-LY)

The product is antibody recognizes EIF3K. The antibody EG1068 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:40000.
See all EIF3K antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse

Clone

EG1068

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 ELISA: 1:40000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human EIF3K.

Specificity

Human, Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Eukaryotic Translation Initiation Factor 3 Subunit K

Introduction

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM, Mar 2008]

Entrez Gene ID

Human	27335
Mouse	73830

UniProt ID

Human	Q9UBQ5
Mouse	Q9DBZ5

Alternative Names

Eukaryotic Translation Initiation Factor 3 Subunit K; Eukaryotic Translation Initiation Factor 3, Subunit 12; Muscle-Specific Gene M9 Protein; EIF-3 P28; EIF3S12; PLAC-24; Eukaryotic Translation Initiation Factor 3, Subunit K; Eukaryotic Translation Initiation Factor 3 Subunit 12; Muscle Specific; EIF-3 P25;

Research Area

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815).

The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632).

The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Biological Process

Formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
Regulation of translational initiation Source: InterPro
Translational initiation Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm

References

Chen, Y., Cao, B., Zheng, W., Sun, Y., & Xu, T. (2022). eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish. Journal of Biological Chemistry, 298(3).

Duan, H., Zhang, S., Zarai, Y., Öllinger, R., Wu, Y., Sun, L., ... & Wolf, D. A. (2022). eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content. bioRxiv.

Lin, L., Cao, J., Du, A., An, Q., Chen, X., Yuan, S., ... & Norvienyeku, J. (2021). eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae. Frontiers in plant science, 12, 748120.

Lin, L., Du, A., An, Q., Chen, X., Yuan, S., Batool, W., ... & Norvienyeku, J. (2021). Corrigendum: eIF3k domain-containing protein regulates conidiogenesis, appressorium turgor, virulence, stress tolerance, physiological and pathogenic development of Magnaporthe oryzae Oryzae. Frontiers in Plant Science, 2790.

Yao, L., Xu, B., & Li, X. (2020). Neisseria gonorrhoeae-induced salpingitis is targeted by circular RNA EIF3K via miR-139-5p and regulating MAPK/NF-κB signaling pathway to promotes apoptosis and autophagy bacterial cells. Microbial pathogenesis, 142, 104051.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-EIF3K Recombinant Antibody (2313C2a)

Mouse Anti-EIF3K Recombinant Antibody (CBFYE-0689)

Mouse Anti-EIF3K Recombinant Antibody (CBFYE-0690)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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