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Mouse Anti-EIF4B Recombinant Antibody (355CT12.6.5) (CBMAB-E0191-FY)

This product is mouse antibody that recognizes EIF4B. The antibody 355CT12.6.5 can be used for immunoassay techniques such as: ELISA, WB.
See all EIF4B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
355CT12.6.5
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
EIF4B Mab is generated from mouse immunized with EIF4B recombinant protein
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 4B
Introduction
EIF4B (Eukaryotic Translation Initiation Factor 4B) is a Protein Coding gene. Among its related pathways are Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S and Viral mRNA Translation. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA binding. An important paralog of this gene is EIF4H.
Entrez Gene ID
1975
UniProt ID
P23588
Alternative Names
Eukaryotic Translation Initiation Factor 4B; EIF-4B; PRO1843
Research Area
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Biological Process
Eukaryotic translation initiation factor 4F complex assembly Source: GO_Central
Formation of translation preinitiation complex Source: GO_Central
Regulation of translational initiation Source: ProtInc
Cellular Location
Cytosol; Dendrite; Eukaryotic translation initiation factor 4F complex; Neuronal cell body; Postsynapse
PTM
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.

Yin, S., Chen, Y., Tong, H., Li, T., Qin, Z., Zhu, J., & He, W. (2022). PP2A promotes apoptosis and facilitates docetaxel sensitivity via the PP2A/p‑eIF4B/XIAP signaling pathway in prostate cancer. Oncology Letters, 23(3), 1-9.

Bettegazzi, B., Sebastian Monasor, L., Bellani, S., Codazzi, F., Restelli, L. M., Colombo, A. V., ... & Zacchetti, D. (2021). Casein Kinase 2 dependent phosphorylation of eIF4B regulates BACE1 expression in Alzheimer’s disease. Cell death & disease, 12(8), 1-14.

Iwao, Y., Okamoto, Y., Shirahama, H., Tsukahara, S., & Tomida, A. (2021). eIF4B enhances ATF4 expression and contributes to cellular adaptation to asparagine limitation in BRAF-mutated A375 melanoma. Biochemical And Biophysical Research Communications, 573, 93-99.

Sasaki, K., Mizuno, H., Ogawa, T., Masamoto, Y., & Kurokawa, M. (2021). eIF4B Is a Novel Target of CAMK2G and Promotes Proliferation of Malignant Cells in Myelofibrosis. Blood, 138, 3586.

Xu, J., Lu, Y., Liu, Q., Xia, A., Zhao, J., Xu, X., ... & Sun, B. (2020). Long noncoding RNA GMAN promotes hepatocellular carcinoma progression by interacting with eIF4B. Cancer letters, 473, 1-12.

Ma, Y., Li, T., Feng, R., Guo, G., Pan, Q., Li, J., & Chen, J. (2020). Synergistic role of JAK/STAT5 and PI3K/AKT signaling pathways in regulating eIF4B in acute leukemia. Sheng wu Gong Cheng xue bao= Chinese Journal of Biotechnology, 36(11), 2413-2423.

Watanabe, D., Nogami, A., Okada, K., Akiyama, H., Umezawa, Y., & Miura, O. (2019). FLT3-ITD Activates RSK1 to Enhance Proliferation and Survival of AML Cells by Activating mTORC1 and eIF4B Cooperatively with PIM or PI3K and by Inhibiting Bad and BIM. Cancers, 11(12), 1827.

Goodman, L. D., Prudencio, M., Srinivasan, A. R., Rifai, O. M., Lee, V. M. Y., Petrucelli, L., & Bonini, N. M. (2019). eIF4B and eIF4H mediate GR production from expanded G4C2 in a Drosophila model for C9orf72-associated ALS. Acta neuropathologica communications, 7(1), 1-16.

Kapadia, B., Nanaji, N. M., Bhalla, K., Bhandary, B., Lapidus, R., Beheshti, A., ... & Gartenhaus, R. B. (2018). Fatty acid synthase induced S6Kinase facilitates USP11-eIF4B complex formation for sustained oncogenic translation in DLBCL. Nature communications, 9(1), 1-15.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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