Sign in or Register   Sign in or Register
  |  

Mouse Anti-EIF4ENIF1 Recombinant Antibody (2314C3a) (CBMAB-E1163-FY)

This product is mouse antibody that recognizes EIF4ENIF1. The antibody 2314C3a can be used for immunoassay techniques such as: DB, ICC, IF, WB.
See all EIF4ENIF1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2314C3a
Antibody Isotype
IgG1
Application
DB, ICC, IF, WB

Basic Information

Immunogen
EIF4 ENIF1 antibody was raised in mouse using recombinant Human Eukaryotic Translation Initiation Factor 4 Nuclear Import Factor 1
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.05% Sodium azide
Concentration
0.1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
eukaryotic translation initiation factor 4E nuclear import factor 1
Introduction
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
56478
UniProt ID
Q9NRA8
Alternative Names
Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1; EIF4E Transporter; 4E-T; Eukaryotic Translation Initiation Factor 4E Transporter; 2610509L04Rik; Clast4
Research Area
EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:27342281, PubMed:32354837).

Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:24335285, PubMed:32354837).

Acts as a binding platform for multiple RNA-binding proteins: promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation (PubMed:27342281, PubMed:32354837).

Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity).

Promotes miRNA-mediated translational repression (PubMed:24335285, PubMed:27342281, PubMed:28487484).

Required for the formation of P-bodies (PubMed:16157702, PubMed:22966201, PubMed:27342281, PubMed:32354837).

Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay (PubMed:32354837).

Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism (PubMed:10856257, PubMed:28216671).
Biological Process
Gene silencing by RNA Source: UniProtKB-KW
mRNA stabilization Source: UniProtKB
Negative regulation of deadenylation-dependent decapping of nuclear-transcribed mRNA Source: UniProtKB
Negative regulation of neuron differentiation Source: Ensembl
Negative regulation of translation Source: UniProtKB
P-body assembly Source: UniProtKB
Positive regulation of miRNA mediated inhibition of translation Source: UniProtKB
Positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
Protein import into nucleus Source: UniProtKB
Stem cell population maintenance Source: Ensembl
Cellular Location
Nucleus; PML body; Nucleus speckle; Cytoplasm; P-body. Predominantly cytoplasmic (PubMed:10856257). Mainly localizes to processing bodies (P-bodies) (PubMed:16157702). Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner (PubMed:10856257). Localization to nuclear foci and speckles requires active transcription (PubMed:22090346).
PTM
Phosphorylation by MAPK8/JNK1 and or MAPK9/JNK2 in response to oxidative stress promotes P-body assembly (PubMed:22966201). Phosphorylated during meiotic maturation (By similarity).
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-EIF4ENIF1 Recombinant Antibody (2314C3a)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare