Mouse Anti-EIF4ENIF1 Recombinant Antibody (CBFYE-0188) (CBMAB-E0431-FY)

Name: Mouse Anti-EIF4ENIF1 Recombinant Antibody (CBFYE-0188)
SKU: CBMAB-E0431-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EIF4ENIF1 Recombinant Antibody (CBFYE-0188) (CBMAB-E0431-FY-1) in Immunoblot and IP

The eIF4E2-MEST/4E-T/GLGYF2 interaction is decreased in HTR-8/SVneo cells transfected with MEST mut, compared with MEST cDNA transfection. eIF4E2 co-IP was performed, and the immunoprecipitated fractions were analyzed by immunoblotting for the indicated proteins. ...View More

Wang, H., Cui, X., Wang, L., Fan, N., Yu, M., Qin, H., ... & Yan, Q. (2023). α1, 3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation. Cell Death & Disease, 14(10), 651.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0188

Application

WB, IP, ELISA

Immunogen

Recombinant of human 4E-T protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 886-986

More Infomation

Target

Full Name

eukaryotic translation initiation factor 4E nuclear import factor 1

Introduction

The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

56478

UniProt ID

Q9NRA8

Alternative Names

Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1; EIF4E Transporter; 4E-T; Eukaryotic Translation Initiation Factor 4E Transporter; 2610509L04Rik; Clast4

Research Area

EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:27342281, PubMed:32354837).

Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:24335285, PubMed:32354837).

Acts as a binding platform for multiple RNA-binding proteins: promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation (PubMed:27342281, PubMed:32354837).

Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity).

Promotes miRNA-mediated translational repression (PubMed:24335285, PubMed:27342281, PubMed:28487484).

Required for the formation of P-bodies (PubMed:16157702, PubMed:22966201, PubMed:27342281, PubMed:32354837).

Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay (PubMed:32354837).

Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism (PubMed:10856257, PubMed:28216671).

Biological Process

Gene silencing by RNA Source: UniProtKB-KW
mRNA stabilization Source: UniProtKB
Negative regulation of deadenylation-dependent decapping of nuclear-transcribed mRNA Source: UniProtKB
Negative regulation of neuron differentiation Source: Ensembl
Negative regulation of translation Source: UniProtKB
P-body assembly Source: UniProtKB
Positive regulation of miRNA mediated inhibition of translation Source: UniProtKB
Positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
Protein import into nucleus Source: UniProtKB
Stem cell population maintenance Source: Ensembl

Cellular Location

Nucleus; PML body; Nucleus speckle; Cytoplasm; P-body. Predominantly cytoplasmic (PubMed:10856257). Mainly localizes to processing bodies (P-bodies) (PubMed:16157702). Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner (PubMed:10856257). Localization to nuclear foci and speckles requires active transcription (PubMed:22090346).

PTM

Phosphorylation by MAPK8/JNK1 and or MAPK9/JNK2 in response to oxidative stress promotes P-body assembly (PubMed:22966201). Phosphorylated during meiotic maturation (By similarity).

Shang, L., Ren, S., Yang, X., Zhang, F., Jin, L., Zhang, X., & Wu, Y. (2022). EIF4ENIF1 variants in two patients with non-syndromic premature ovarian insufficiency. European Journal of Medical Genetics, 104597.

Moriwaki, M., & Welt, C. K. (2021). Heterozygous Eif4nif1 Stop Gain Mice Replicate the Primary Ovarian Insufficiency Phenotype. Journal of the Endocrine Society, 5(Suppl 1), A774.

Zhao, M., Feng, F., Chu, C., Yue, W., & Li, L. (2019). A novel EIF4ENIF1 mutation associated with a diminished ovarian reserve and premature ovarian insufficiency identified by whole-exome sequencing. Journal of ovarian research, 12(1), 1-6.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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