Rabbit Anti-ENAH Monoclonal Antibody (EPR5660) (CBMAB-1145-YC)

Provided herein is a rabbit monoclonal antibody against Human ENAH. The antibody, clone EPR5660, can be used for immunoassay techniques, such as IHC-P, FC, Cyt and WB.
See all ENAH antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse

Clone

EPR5660

Antibody Isotype

IgG

Application

IHC-P, FC, Cyt, WB

Basic Information

Immunogen

Synthetic peptide corresponding to a sequence within Human ENAH (Q8N8S7)

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

ENAH, Actin Regulator

Introduction

ENAH is a member of the enabled/ vasodilator-stimulated phosphoprotein, which are involved in actin-based motility. ENAH is involved in regulating the assembly of actin filaments and modulates cell adhesion and motility.

Entrez Gene ID

Human	55740
Mouse	13800

UniProt ID

Human	Q8N8S7
Mouse	Q03173

Alternative Names

ENA; MENA; NDPP1

Research Area

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity).

Biological Process

Actin polymerization-dependent cell motility Source: GO_Central
Actin polymerization or depolymerization Source: GO_Central
Axon guidance Source: GO_Central

Cellular Location

Cytoskeleton; Cytoplasm; Lamellipodium; Filopodium; Synapse; Focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses (By similarity).

PTM

NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.

References

Hwang, T., Parker, S. S., Hill, S. M., Grant, R. A., Ilunga, M. W., Sivaraman, V., ... & Keating, A. E. (2022). Native proline-rich motifs exploit sequence context to target actin-remodeling Ena/VASP protein ENAH. ELife, 11, e70680.

Hwang, T. (2022). How short, degenerate motifs across the human proteome recognize the actin remodeling factor ENAH (Doctoral dissertation, Massachusetts Institute of Technology).

Yang, X., Li, Y., Wu, D., Ma, Y., & Zhou, S. (2022). Screening and identification of key genes ATP1B3 and ENAH in the progression of hepatocellular carcinoma: based on data mining and clinical validation. Nan Fang yi ke da xue xue bao= Journal of Southern Medical University, 42(6), 815-823.

Deng, G., Luo, Y., Zhang, Y., Zhang, J., & He, Z. (2022). Enabled homolog (ENAH) regulated by RNA binding protein splicing factor 3b subunit 4 (SF3B4) exacerbates the proliferation, invasion and migration of hepatocellular carcinoma cells via Notch signaling pathway. Bioengineered, 13(2), 2194-2206.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Rabbit Anti-ENAH Recombinant Antibody (CBFYM-1082)

Rabbit Anti-ENAH Recombinant Antibody (EG1108)

Mouse Anti-ENAH Recombinant Antibody (3E6)

Mouse Anti-ENAH (AA 291-391) Recombinant Antibody (CBFYE-0210)

Mouse Anti-ENAH (AA 415-541) Recombinant Antibody (21/Mena)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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