Sign in or Register   Sign in or Register
  |  

Mouse Anti-ERAP1 Recombinant Antibody (4H8) (CBMAB-A0506-LY)

The product is antibody recognizes ARTS-1. The antibody 4H8 immunoassay techniques such as: WB, ELISA.
See all ERAP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4H8
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
ARTS-1 (AAH30775, 832 a.a. ~ 941 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Endoplasmic Reticulum Aminopeptidase 1
Introduction
Aminopeptidases play a role in the metabolism of several peptides that may be involved in blood pressure and the pathogenesis of essential hypertension (MIM 145500). Adipocyte-derived leucine aminopeptidase (ALAP) is a member of the M1 family of zinc metallopeptidases.[supplied by OMIM
Entrez Gene ID
51752
UniProt ID
Q9NZ08
Alternative Names
A-LAP; ALAP; APPILS; ARTS-1; ARTS1; ERAAP; ERAAP1; KIAA0525; PILS-AP; PILSAP
Research Area
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
Biological Process
Adaptive immune response Source: UniProtKB-KW
Angiogenesis Source: HGNC-UCL
Antigen processing and presentation of endogenous peptide antigen via MHC class I Source: HGNC-UCL
Antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
Fat cell differentiation Source: UniProtKB
Membrane protein ectodomain proteolysis Source: UniProtKB
Peptide catabolic process Source: GO_Central
Positive regulation of angiogenesis Source: Ensembl
Proteolysis Source: GO_Central
Regulation of blood pressure Source: GO_Central
Regulation of innate immune response Source: UniProtKB
Response to bacterium Source: BHF-UCL
Signal transduction Source: GO_Central
Cellular Location
Endoplasmic reticulum membrane
Topology
Cytoplasmic: 1
Helical: 2-21
Lumenal: 22-941
PTM
N-glycosylated.

Arakawa, A., Reeves, E., Vollmer, S., Arakawa, Y., He, M., Galinski, A., ... & Prinz, J. C. (2021). ERAP1 controls the autoimmune response against melanocytes in psoriasis by generating the melanocyte autoantigen and regulating its amount for HLA-C* 06: 02 presentation. The Journal of Immunology, 207(9), 2235-2244.

Maben, Z., Arya, R., Georgiadis, D., Stratikos, E., & Stern, L. J. (2021). Conformational dynamics linked to domain closure and substrate binding explain the ERAP1 allosteric regulation mechanism. Nature communications, 12(1), 1-15.

Reeves, E., Islam, Y., & James, E. (2020). ERAP1: A potential therapeutic target for a myriad of diseases. Expert Opinion on Therapeutic Targets, 24(6), 535-544.

Babaie, F., Hosseinzadeh, R., Ebrazeh, M., Seyfizadeh, N., Aslani, S., Salimi, S., ... & Mohammadi, H. (2020). The roles of ERAP1 and ERAP2 in autoimmunity and cancer immunity: New insights and perspective. Molecular immunology, 121, 7-19.

Bufalieri, F., Infante, P., Bernardi, F., Caimano, M., Romania, P., Moretti, M., ... & Di Marcotullio, L. (2019). ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. Nature communications, 10(1), 1-15.

Compagnone, M., Cifaldi, L., & Fruci, D. (2019). Regulation of ERAP1 and ERAP2 genes and their disfunction in human cancer. Human immunology, 80(5), 318-324.

Admon, A. (2019). ERAP1 shapes just part of the immunopeptidome. Human immunology, 80(5), 296-301.

Yao, Y., Liu, N., Zhou, Z., & Shi, L. (2019). Influence of ERAP1 and ERAP2 gene polymorphisms on disease susceptibility in different populations. Human immunology, 80(5), 325-334.

Reeves, E., & James, E. (2018). The role of polymorphic ERAP1 in autoinflammatory disease. Bioscience reports, 38(4).

López de Castro, J. A. (2018). How ERAP1 and ERAP2 shape the peptidomes of disease-associated MHC-I proteins. Frontiers in Immunology, 9, 2463.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ERAP1 Recombinant Antibody (4H8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare