Rabbit Anti-ERGIC3 Recombinant Antibody (EG1137) (CBMAB-EN1354-LY)

The product is antibody recognizes ERGI3. The antibody EG1137 immunoassay techniques such as: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000.
See all ERGIC3 antibodies

Summary

Host Animal

Rabbit

Specificity

Human, Mouse

Clone

EG1137

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human ERGI3.

Specificity

Human, Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Ergic And Golgi 3

Entrez Gene ID

Human	51614
Mouse	66366

UniProt ID

Human	Q9Y282
Mouse	Q9CQE7

Research Area

Possible role in transport between endoplasmic reticulum and Golgi.

Biological Process

Endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: GO_Central

Cellular Location

Cis-Golgi network membrane; Endoplasmic reticulum membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane. Cycles between the endoplasmic reticulum and the Golgi.

Topology

Cytoplasmic: 1-25
Helical: 26-46
Lumenal: 47-341
Helical: 342-362
Cytoplasmic: 363-383

References

Guan, L., Yang, Y., Liang, J., Miao, Y., Shang, A., Wang, B., ... & Ding, M. (2022). ERGIC2 and ERGIC3 regulate the ER‐to‐Golgi transport of gap junction proteins in metazoans. Traffic, 23(3), 140-157.

Liu, M., Zhao, Q., Zheng, X., Yang, L., Zhao, Y., Li, X., & Wu, M. (2022). Transcriptome changes in ERGIC3-knockdown hepatocellular carcinoma cells: ERGIC3 is a novel immune function related gene. PeerJ, 10, e13369.

Zhao, Q., Wu, M., Zheng, X., Yang, L., Zhang, Z., Li, X., & Chen, J. (2020). ERGIC3 silencing additively enhances the growth inhibition of BFA on lung adenocarcinoma cells. Current Cancer Drug Targets, 20(1), 67-75.

Yoo, W., Cho, E. B., Kim, S., & Yoon, J. B. (2019). The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins. Journal of Biological Chemistry, 294(28), 10900-10912.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ERGIC3 Recombinant Antibody (CBFYE-1191)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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