Sign in or Register   Sign in or Register
  |  

Mouse Anti-ESPL1 Recombinant Antibody (6H6) (CBMAB-A2717-LY)

The product is antibody recognizes ESPL1. The antibody 6H6 immunoassay techniques such as: WB, ELISA.
See all ESPL1 antibodies
Published Data
Fig.1 Examples of separase immunoexpression in invasive breast carcinomas and benign breast epithelium.
...View More
Fig.2 Immunofluorescence images show overexpression of Separase protein in primary glioblastoma cells.
...View More
Fig.3 Western Blot shows overexpression of Separase protein in primary adult glioblastoma tissues.
...View More

Summary

Host Animal
Mouse
Specificity
Human
Clone
6H6
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
ESPL1 (NP_036423, 586 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
extra spindle pole bodies homolog 1 (S. cerevisiae)
Introduction
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 604359) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM
Entrez Gene ID
9700
UniProt ID
Q14674
Alternative Names
ESP1; FLJ46492; KIAA0165; SEPARASE; SEPARIN
Research Area
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
Biological Process
Apoptotic process Source: UniProtKB
Establishment of mitotic spindle localization Source: UniProtKB
Homologous chromosome segregation Source: Ensembl
Meiotic chromosome separation Source: GO_Central
Meiotic spindle organization Source: Ensembl
Mitotic cytokinesis Source: UniProtKB
Mitotic sister chromatid segregation Source: UniProtKB
Negative regulation of sister chromatid cohesion Source: UniProtKB
Positive regulation of mitotic metaphase/anaphase transition Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm
PTM
Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ESPL1 Recombinant Antibody (6H6)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare