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Mouse Anti-ESPL1 Recombinant Antibody (XJ11-1B12) (CBMAB-S0194-CQ)

This product is a mouse antibody that recognizes ESPL1. The antibody XJ11-1B12 can be used for immunoassay techniques such as: WB, FC, IF.
See all ESPL1 antibodies
Published Data
Fig.1 Analysis of separase localization by cellular fractionation.
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Fig.2 Immunofluorescent staining of mitotic chromosome spreads.
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Fig.1 HeLa cells were synchronously released from G1/S phase in the presence of RO3306 (10 μM) or STLC (10 μM).
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Fig.2 Leaky APC/C and separase activity drives centrosome fragmentation and premature centriole disengagement during mitotic delay.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
XJ11-1B12
Antibody Isotype
IgG1, κ
Application
WB, FC, IF

Basic Information

Immunogen
Maltose-Binding Protein fusion of a C-terminal fragment of human Separase (residues 1866-1996). [UniProt# Q14674]
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
0.9 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
extra spindle pole bodies homolog 1 (S. cerevisiae)
Introduction
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).
Entrez Gene ID
9700
UniProt ID
Q14674
Alternative Names
Extra Spindle Pole Bodies Like 1, Separase; Extra Spindle Poles-Like 1 Protein; Separin, Cysteine Protease; Caspase-Like Protein ESPL1; Separase; Separin; ESP1; Extra Spindle Pole Bodies Homolog 1 (S. Cerevisiae);
Research Area
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
Biological Process
Apoptotic process Source: UniProtKB
Establishment of mitotic spindle localization Source: UniProtKB
Homologous chromosome segregation Source: Ensembl
Meiotic chromosome separation Source: GO_Central
Meiotic spindle organization Source: Ensembl
Mitotic cytokinesis Source: UniProtKB
Mitotic sister chromatid segregation Source: UniProtKB
Negative regulation of sister chromatid cohesion Source: UniProtKB
Positive regulation of mitotic metaphase/anaphase transition Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm
PTM
Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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