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Mouse Anti-ETV4 (AA 1-207) Recombinant Antibody (3G9-1B9) (CBMAB-E1973-FY)

This product is mouse antibody that recognizes ETV4. The antibody 3G9-1B9 can be used for immunoassay techniques such as: ELISA, WB.
See all ETV4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3G9-1B9
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
ETV4 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: MYLHTEGFSG PSPGDGAMGY GYEKPLRPFP DDVCVAPEKF EGDIKQEGVG AFREGPPYQR RGALQLWQFL VALLDDPTNA HFIAWTGRGM EFKLIEPEEV ARLWGIQKNR PAMNYDKLSR SLRYYYEKGI MQKVAGERYV YKFVCEPEAL FSLAFPDNQR PALKAEFDRP VSEEDTVPLS HLDESPAYLP ELAGPAQPFG PKGGYSY
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-207

Target

Full Name
ETS Variant 4
Introduction
ETV4L (ETS Variant 4 Like) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and RNA polymerase II transcription factor activity, sequence-specific DNA binding. An important paralog of this gene is ETV3.
Entrez Gene ID
UniProt ID
Alternative Names
ETS Variant 4; Ets Variant Gene 4 (E1A Enhancer-Binding Protein, E1AF); Polyomavirus Enhancer Activator 3 Homolog; Adenovirus E1A Enhancer-Binding Protein; E1A-F; E1AF; PEA3
Research Area
Transcriptional activator (PubMed:19307308, PubMed:31552090).

May play a role in keratinocyte differentiation (PubMed:31552090).

(Microbial infection) Binds to the enhancer of the adenovirus E1A gene and acts as a transcriptional activator; the core-binding sequence is 5'-[AC]GGA[AT]GT-3'.
Biological Process
Cell differentiation Source: GO_Central
Positive regulation of keratinocyte differentiation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
PTM
Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.

Wang, J., Sun, C., Li, J., Jiang, H., Qiu, Y., & Gong, M. (2022). Knockdown of ETV4 promotes autophagy‑dependent apoptosis in GBM cells by reducing the transcriptional activation of EMP1. Oncology Letters, 23(2), 1-10.

IV, A. N., & Nair, A. S. (2022). Bioinformatics screening of ETV4 transcription factor oncogenes and identifying small‐molecular anticancer drugs. Chemical Biology & Drug Design, 99(2), 277-285.

Wang, L., Zhang, Y., Yang, J., Liu, L., Yao, B., Tian, Z., & He, J. (2021). The Knockdown of ETV4 Inhibits the Papillary Thyroid Cancer Development by Promoting Ferroptosis Upon SLC7A11 Downregulation. DNA and Cell Biology, 40(9), 1211-1221.

Yao, D., Bao, Z., Qian, X., Yang, Y., & Mao, Z. (2021). ETV4 transcriptionally activates HES1 and promotes Stat3 phosphorylation to promote malignant behaviors of colon adenocarcinoma. Cell Biology International, 45(10), 2129-2139.

Yatagai, Y., Oshima, H., Sakamoto, T., Shigemasa, R., Kitazawa, H., Hyodo, K., ... & Hizawa, N. (2021). Expression quantitative trait loci for ETV4 and MEOX1 are associated with adult asthma in Japanese populations. Scientific reports, 11(1), 1-9.

Wang, B., Cai, Y., Li, X., Kong, Y., Fu, H., & Zhou, J. (2021). ETV4 mediated lncRNA C2CD4D-AS1 overexpression contributes to the malignant phenotype of lung adenocarcinoma cells via miR-3681-3p/NEK2 axis. Cell Cycle, 20(24), 2607-2618.

Wang, Y., Ding, X., Liu, B., Li, M., Chang, Y., Shen, H., ... & Li, Y. (2020). ETV4 overexpression promotes progression of non–small cell lung cancer by upregulating PXN and MMP1 transcriptionally. Molecular Carcinogenesis, 59(1), 73-86.

Deshmukh, S. K., Singh, A. P., & Singh, S. (2018). ETV4: an emerging target in pancreatic cancer. Oncoscience, 5(9-10), 260.

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For research use only. Not intended for any clinical use.

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