Mouse Anti-EYA3 Recombinant Antibody (CBFYE-1426) (CBMAB-E2051-FY)

This product is mouse antibody that recognizes EYA3. The antibody CBFYE-1426 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all EYA3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYE-1426

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.2 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Eya Transcriptional Coactivator And Phosphatase 3

Introduction

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.

Entrez Gene ID

2140

UniProt ID

Q99504

Alternative Names

EYA Transcriptional Coactivator And Phosphatase 3; EC 3.1.3.48; Eyes Absent (Drosophila) Homolog 3; Eyes Absent Homolog 3 (Drosophila)

Research Area

Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 (PubMed:19234442, PubMed:19351884).

Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity).

May be involved in development of the eye.

Biological Process

Anatomical structure development Source: GO_Central
Anatomical structure morphogenesis Source: ProtInc
Cell differentiation Source: GO_Central
Double-strand break repair Source: UniProtKB
Histone dephosphorylation Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
Positive regulation of DNA repair Source: UniProtKB
Response to ionizing radiation Source: UniProtKB
Visual perception Source: ProtInc

Cellular Location

Nucleus; Cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:19234442). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively (By similarity).

PTM

Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.

References

Yang, C., & Liu, H. (2022). Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression. Annals of translational medicine, 10(13).

Xu, S., Lian, Z., Zhang, S., Xu, Y., & Zhang, H. (2021). CircGNG4 promotes the progression of prostate cancer by sponging miR-223 to enhance EYA3/c-myc expression. Frontiers in Cell and Developmental Biology, 9.

Ionescu, A. E., Mentel, M., Munteanu, C. V., Sima, L. E., Martin, E. C., Necula-Petrareanu, G., & Szedlacsek, S. E. (2019). Analysis of EYA3 phosphorylation by Src kinase identifies residues involved in cell proliferation. International journal of molecular sciences, 20(24), 6307.

Wang, Y., Pandey, R. N., York, A. J., Mallela, J., Nichols, W. C., Hu, Y. C., ... & Hegde, R. S. (2019). The EYA3 tyrosine phosphatase activity promotes pulmonary vascular remodeling in pulmonary arterial hypertension. Nature communications, 10(1), 1-13.

Vartuli, R. L., Zhou, H., Zhang, L., Powers, R. K., Klarquist, J., Rudra, P., ... & Ford, H. L. (2018). Eya3 promotes breast tumor–associated immune suppression via threonine phosphatase–mediated PD-L1 upregulation. The Journal of clinical investigation, 128(6), 2535-2550.

Zhang, L., Zhou, H., Li, X., Vartuli, R. L., Rowse, M., Xing, Y., ... & Ford, H. L. (2018). Eya3 partners with PP2A to induce c-Myc stabilization and tumor progression. Nature communications, 9(1), 1-14.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-EYA3 Recombinant Antibody (CBFYE-1426)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: